Plant growth promoting rhizobacteria (PGPR) are important for agriculture through their activity in stimulating and facilitating plant growth. The rhizobacteria were screened for molecular characterization and followed by their indole acetic acid (IAA) production, phosphate solubility, antibiosis activity. In this study, 162 soil samples were collected from the cocoa rhizosphere to isolate Bacillus subtilis strains using Mössel agar medium with an additional egg yolk and identified by sequencing the ytcP gene. The ability of each strain to form biofilms was obtained in a tube. Indole-3-acetic acid (IAA) production was estimated in Yeast Peptone Dextrose (YPB) broth. Phosphates were solubilized by each strain on Pikovskaya agar medium. The detection of lipopeptide genes using the molecular method has established the possession of isolates by antimicrobial genes. Fifty (50) B. subtilis strains were isolated and identified using the ytcP gene. Ninety percent (90%) of the strains were able to form a biofilm. All isolates produced an IAA. Forty (40 (80%)) of B. subtilis were solubilized phosphate with phosphate solubilizing index (PSI) of 0 to 97.33 ± 0.70%. Of all B. subtilis strains, 45 (90%) have the srfAA gene, 19 (38%) have the fenD gene and 12 (24%) have the ituC gene. B. subtilis strains from cocoa rhizosphere would be beneficial for agricultural production by their PGPR activities.

中文翻译：

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从可可树 (Theobroma cacao L.) 根际分离的枯草芽孢杆菌菌株用作科特迪瓦潜在的促进植物生长的根际细菌

植物生长促进根际细菌 (PGPR) 通过它们刺激和促进植物生长的活动对农业很重要。筛选根际细菌的分子特征，然后是它们的吲哚乙酸 (IAA) 产量、磷酸盐溶解度、抗菌活性。在这项研究中，从可可根际收集了 162 个土壤样品，以使用带有额外蛋黄的 Mössel 琼脂培养基分离枯草芽孢杆菌菌株，并通过对 ytcP 基因进行测序进行鉴定。在管中获得每个菌株形成生物膜的能力。在酵母蛋白胨葡萄糖 (YPB) 肉汤中估计吲哚-3-乙酸 (IAA) 产量。磷酸盐在 Pikovskaya 琼脂培养基上被每个菌株溶解。使用分子方法检测脂肽基因已经确立了抗菌基因对分离株的拥有。使用 ytcP 基因分离并鉴定了五十 (50) 个枯草芽孢杆菌菌株。百分之九十 (90%) 的菌株能够形成生物膜。所有分离株均产生 IAA。四十 (40 (80%)) 种枯草芽孢杆菌是溶解磷酸盐，磷酸盐溶解指数 (PSI) 为 0 至 97.33 ± 0.70%。在所有枯草芽孢杆菌菌株中，45 个 (90%) 具有 srfAA 基因，19 个 (38%) 具有 fenD 基因，12 个 (24%) 具有 ituC 基因。来自可可根际的枯草芽孢杆菌菌株通过其 PGPR 活动有利于农业生产。四十 (40 (80%)) 种枯草芽孢杆菌是溶解磷酸盐，磷酸盐溶解指数 (PSI) 为 0 至 97.33 ± 0.70%。在所有枯草芽孢杆菌菌株中，45 个 (90%) 具有 srfAA 基因，19 个 (38%) 具有 fenD 基因，12 个 (24%) 具有 ituC 基因。来自可可根际的枯草芽孢杆菌菌株通过其 PGPR 活动有利于农业生产。四十 (40 (80%)) 种枯草芽孢杆菌是溶解磷酸盐，磷酸盐溶解指数 (PSI) 为 0 至 97.33 ± 0.70%。在所有枯草芽孢杆菌菌株中，45 个 (90%) 具有 srfAA 基因，19 个 (38%) 具有 fenD 基因，12 个 (24%) 具有 ituC 基因。来自可可根际的枯草芽孢杆菌菌株通过其 PGPR 活动有利于农业生产。