L-asparaginases are extensively applied in the treatment of Acute Lymphoblastic Leukemia (ALL). The treatment regime of ALL consists of asparaginase from E. coli or Erwinia. The survival rate post-chemotherapy has increased to < 90% in recent years. Asparaginase therapy has also resulted in numerous toxicities to patients receiving therapy. This study demonstrates the reaction of normal cells of Danio rerio to asparaginase therapy. L-asparaginase I and II used in the present study are from two probiotic Lactobacillus species in comparison with a commercial L-asparaginase of E. coli origin. Zebrafish adults were injected with 2500 U/kg body weight of L-asparaginase treatments. The expression of SOD 2, CAT, GST, GTP BP3, FADS2 were analyzed with EF1α as house-keeping gene. The p value obtained proves that the data are significant. The histology of the L-asparaginase I treated fishes showed dilated sinusoids in the liver and pseudocyst in the pancreas. The L-asparaginase II and commercial asparaginase showed no pathology.

L-天冬酰胺酶广泛应用于治疗急性淋巴细胞白血病 (ALL)。ALL 的治疗方案包括来自大肠杆菌或欧文氏菌的天冬酰胺酶。近年来，化疗后的存活率已增加到 < 90%。天冬酰胺酶治疗也对接受治疗的患者产生了许多毒性。该研究证明了Danio rerio的正常细胞对天冬酰胺酶治疗的反应。本研究中使用的 L-天冬酰胺酶 I 和 II 来自两种益生菌乳杆菌，与大肠杆菌来源的商业 L-天冬酰胺酶相比。斑马鱼成鱼注射了 2500 U/kg 体重的 L-天冬酰胺酶处理剂。的表达SOD 2 , CAT , GST , GTP BP3 , FADS2以EF1α为管家基因进行分析。得到的p值证明数据是显着的。L-天冬酰胺酶 I 处理的鱼的组织学显示肝脏中扩张的血窦和胰腺中的假性囊肿。L-天冬酰胺酶 II 和商品化天冬酰胺酶未显示病理。