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Toxicity Analysis of Recombinant L-asparaginase I and II in Zebrafish

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Abstract

L-asparaginases are extensively applied in the treatment of Acute Lymphoblastic Leukemia (ALL). The treatment regime of ALL consists of asparaginase from E. coli or Erwinia. The survival rate post-chemotherapy has increased to < 90% in recent years. Asparaginase therapy has also resulted in numerous toxicities to patients receiving therapy. This study demonstrates the reaction of normal cells of Danio rerio to asparaginase therapy. L-asparaginase I and II used in the present study are from two probiotic Lactobacillus species in comparison with a commercial L-asparaginase of E. coli origin. Zebrafish adults were injected with 2500 U/kg body weight of L-asparaginase treatments. The expression of SOD 2, CAT, GST, GTP BP3, FADS2 were analyzed with EF1α as house-keeping gene. The p value obtained proves that the data are significant. The histology of the L-asparaginase I treated fishes showed dilated sinusoids in the liver and pseudocyst in the pancreas. The L-asparaginase II and commercial asparaginase showed no pathology.

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Acknowledgements

The authors thank Dr. S. Iyappan, Dr. S. Kirankumar and research scholars of Zebrafish genetics lab for their help and support. Authors thank the SRM-DBT Partnership Platform for Contemporary Research Services and Skill Development in Advanced Life Sciences Technologies (No. BT/PR12987/INF/22/205/2015) for providing the RT-PCR facility. The authors thank Dr. Usha Rani Ravikumar, Professor, and Head of Pathology, Velammal Medical College for analyzing the histology slides.

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  1. Department of Genetic Engineering, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India

    Susan Aishwarya Suresh, Selvarajan Ethiraj & K. N. Rajnish

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  1. Susan Aishwarya Suresh
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  2. Selvarajan Ethiraj
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  3. K. N. Rajnish
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Correspondence to Selvarajan Ethiraj or K. N. Rajnish.

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Suresh, S.A., Ethiraj, S. & Rajnish, K.N. Toxicity Analysis of Recombinant L-asparaginase I and II in Zebrafish. Indian J Microbiol 60, 535–538 (2020). https://doi.org/10.1007/s12088-020-00890-7

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