Conventional therapeutic applications of mesenchymal stromal cells (MSCs) focus on cell replacement and differentiation; however, increasing evidence suggests that most of their therapeutic effects are carried out by their various secretions. This study investigated the application of conditioned medium (CM) from human umbilical cord blood-derived MSCs (hUCB-MSCs) to improve hair growth and developed a method to reliably produce this optimized CM. Primed MSC-derived CM (P-CM) with combinations of TGF-β1 and LiCl was optimized by comparing its effects on the cell viability of dermal papilla cells (DPCs). P-CM significantly increased the viability of DPCs compared to CM. The secretion of vascular endothelial growth factor (VEGF) in DPCs was regulated by the macrophage migration inhibitory factor (MIF) in the P-CM secreted by MSCs. These findings suggest that P-CM can improve the efficacy in hair growth via a paracrine mechanism and that MIF in P-CM exerts hair growth-promoting effects via a VEGF-related β-catenin and p-GSK-3β [SER9] signaling pathway. Furthermore, clinical trials have shown that 5% P-CM improved androgenetic alopecia through producing an increased hair density, thickness, and growth rate, suggesting that this topical agent may be a novel and effective treatment option for patients with androgenetic alopecia.

中文翻译：

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人脐带血来源的间充质基质细胞条件培养基中的迁移抑制因子刺激毛发生长。


间充质基质细胞（MSC）的传统治疗应用集中于细胞替代和分化；然而，越来越多的证据表明，它们的大部分治疗作用是通过它们的各种分泌物来实现的。本研究调查了人脐带血间充质干细胞 (hUCB-MSC) 的条件培养基 (CM) 在促进毛发生长方面的应用，并开发了一种可靠地生产这种优化的 CM 的方法。通过比较其对真皮乳头细胞 (DPC) 细胞活力的影响，优化了含有 TGF-β1 和 LiCl 组合的 MSC 衍生 CM (P-CM)。与 CM 相比，P-CM 显着提高了 DPC 的活力。 DPCs中血管内皮生长因子(VEGF)的分泌受到MSCs分泌的P-CM中的巨噬细胞迁移抑制因子(MIF)的调节。这些发现表明，P-CM 可以通过旁分泌机制提高毛发生长的功效，并且 P-CM 中的 MIF 通过 VEGF 相关的 β-catenin 和 p-GSK-3β [SER9] 信号通路发挥促进毛发生长的作用。此外，临床试验表明，5% P-CM 通过增加头发密度、厚度和生长速度来改善雄激素性脱发，表明这种外用制剂可能是雄激素性脱发患者的一种新颖且有效的治疗选择。