Defense proteome of sugarcane in response to red rot pathogen Colletotrichum falcatum was established for the first time upon analyzing the differentially expressed proteins of resistant and susceptible cultivars through two dimensional gel electrophoresis (2-DE) and mass spectrometry. The proteomes of red rot resistant (Co 93009) and susceptible varieties (CoC 671) showed significant differences in their protein expression after pathogen inoculation. The resistant variety showed a dynamic activation and regulation of cellular components upon infection from the pathogen, whereas the response was submissive in the susceptible variety. A total of 136 differentially expressed proteins were characterized by MALDI-TOF and the identified proteins were grouped into seven broad categories based on their putative functions. The major portion of the up-regulated proteins belonged to signal transduction, transcription/ transcription factors, metabolism, defense/ stress and retroelements. Among the down-regulated proteins, majority belonged to metabolism, cell growth and development, followed by signal transduction and transcription factors. The important proteins identified by MALDI-TOF analyses were of those involved in various defense/ cellular functions such as cyclic nucleotide gated ion channel protein, callose synthase, R2R3-MYB transcription factor MYB6, WRKY transcription factor, putative p-coumarate 3-hydroxylase, PISTILLATA-like protein, flagellar associated protein, class III HD-Zip protein HDZ34, calcium dependent protein kinase, DNAJ heat shock protein, polyphenoloxidase, cytokinin oxidase 3, NB-ARC domain containing protein, NADH dehydrogenase, UDP glucose:flavonoid 3-O-glucosyltransferase, ACC synthase and putative disease resistance protein. The isolation and characterization of differentially expressed proteins would help us to understand the complex host-pathogen interaction at molecular level during the course of disease development/ progression.

中文翻译：

---

鉴定差异表达蛋白并建立甘蔗应对镰状炭疽菌感染的防御蛋白质组

通过二维凝胶电泳（2-DE）和质谱分析抗性和易感品种的差异表达蛋白质，首次建立了甘蔗响应红腐病菌Colletotrichum falcatum的防御蛋白质组。红腐病抗性 (Co 93009) 和易感品种 (CoC 671) 的蛋白质组在病原体接种后显示出显着的蛋白质表达差异。抗性品种在病原体感染后表现出细胞成分的动态激活和调节，而易感品种的反应是顺从的。共有 136 种差异表达的蛋白质通过 MALDI-TOF 进行表征，所鉴定的蛋白质根据其推定的功能分为七大类。上调蛋白的主要部分属于信号转导、转录/转录因子、代谢、防御/应激和逆转录因子。在下调的蛋白质中，大部分属于代谢、细胞生长和发育，其次是信号转导和转录因子。MALDI-TOF 分析鉴定的重要蛋白质是那些参与各种防御/细胞功能的蛋白质，如环核苷酸门控离子通道蛋白、胼胝合酶、R2R3-MYB 转录因子 MYB6、WRKY 转录因子、假定的 p-香豆酸 3-羟化酶、 PISTILLATA 样蛋白、鞭毛相关蛋白、III 类 HD-Zip 蛋白 HDZ34、钙依赖性蛋白激酶、DNAJ 热休克蛋白、多酚氧化酶、细胞分裂素氧化酶 3、含 NB-ARC 结构域的蛋白、NADH 脱氢酶、UDP 葡萄糖：类黄酮 3-O-葡萄糖基转移酶、ACC 合酶和推定的抗病蛋白。差异表达蛋白的分离和表征将帮助我们了解疾病发展/进展过程中分子水平上复杂的宿主-病原体相互作用。