Chitosan films entrapped with the Mansoa hirsuta fraction (CMHF) was developed as a new dressing for wound care. The chromatographic profile of the M. hirsuta fraction (MHF) was evaluated by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, and the results showed that MHF is rich in acid triterpenes. Physicochemical characterization of the films prepared using the solvent casting method was performed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetry (TGA), differential scanning calorimetry (DCS), scanning electron microscopy (SEM), atomic force microscopy (AFM), and mechanical properties. CMHF exhibited characteristic bands of both chitosan and MHF, revealing a physical mixture of both. CMHF presented an amorphous nature, thermostability, and dispersion of MHF in the chitosan matrix, resulting in a rough structure. Incorporation of M. hirsuta fraction into chitosan matrix favorably enhanced the mechanical performance and films thickness. The in vivo wound treatment with CMHF for seven days showed a characteristic area of advanced healing, re-epithelization, cell proliferation, and collagen formation. Furthermore, wound closure reached 100% contraction after 10 days of treatment with modulation of interleukins. The incorporation of M. hirsuta fraction into chitosan films was advantageous and showed great potential for stimulating wound repair and regeneration.

中文翻译：

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壳聚糖膜，包含Mansoa hirsuta馏分用于伤口愈合。

包埋有Mansoa hirsuta馏分（CMHF）的壳聚糖薄膜是一种用于伤口护理的新型敷料。hirsuta M.的色谱图通过超高效液相色谱-四极杆飞行时间质谱法对馏分（MHF）进行了评估，结果表明，MHF中富含酸性三萜烯。使用傅里叶变换红外光谱（FTIR），X射线衍射（XRD），热重分析（TGA），差示扫描量热法（DCS），扫描电子显微镜（SEM），原子分析法对使用溶剂流延法制备的薄膜进行理化表征力显微镜（AFM）和机械性能。CMHF展示了壳聚糖和MHF的特征谱带，揭示了两者的物理混合物。CMHF具有无定形性质，热稳定性和MHF在壳聚糖基质中的分散性，从而导致结构粗糙。hirsuta M.的合并壳聚糖基质中的馏分有利地提高了机械性能和膜厚度。CMHF的体内伤口治疗7天显示出晚期愈合，再上皮形成，细胞增殖和胶原形成的特征区域。此外，用白介素调节治疗10天后伤口闭合达到100％收缩。掺入M. hirsuta的级分分离成脱乙酰壳多糖膜是有利的，并用于刺激伤口修复和再生显示出巨大的潜力。