OsHsfB4d Binds the Promoter and Regulates the Expression of OsHsp18.0-CI to Resistant Against Xanthomonas Oryzae.,Rice

当前位置： X-MOL 学术 › Rice › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

OsHsfB4d Binds the Promoter and Regulates the Expression of OsHsp18.0-CI to Resistant Against Xanthomonas Oryzae.
Rice ( IF 4.8 ) Pub Date : 2020-05-27 , DOI: 10.1186/s12284-020-00388-2
Wei Yang _{1,

2} , Yanhu Ju _{1,

3} , Liping Zuo _{1,

4} , Luyue Shang _{1,

4} , Xinru Li ₃ , Xiaoming Li _{1,

3} , Shangzong Feng ₅ , Xinhua Ding _{1,

4} , Zhaohui Chu _{1,

3}

Affiliation

Background

Bacterial leaf streak (BLS) and bacterial blight (BB) are two major prevalent and devastating rice bacterial diseases caused by the Gram-negative bacteria of Xanthomonas oryzae pv. oryzicola (Xoc) and Xanthomonas oryzae pv. oryzae (Xoo), respectively. Previously, we identified a defence-related (DR) gene encoding a small heat shock protein, OsHsp18.0-CI, that positively regulates BLS and BB resistance in rice.

Results

To reveal the regulatory mechanism of the OsHsp18.0-CI response to Xoc and Xoo, we characterized the class B heat shock factor (Hsf), OsHsfB4d, through transcriptional analysis and a transgenic study. OsHsfB4d is upregulated post inoculation by either the Xoc strain RS105 or Xoo strain PXO99a in Zhonghua 11 (wild type, ZH11) as well as in OsHsp18.0-CI overexpressing rice plants. Transient expression of OsHsfB4d can activate the expression of green fluorescent protein (GFP) and luciferase (Luc) via the OsHsp18.0-CI promoter. Rice plants overexpressing OsHsfB4d exhibited enhanced resistance to RS105 and PXO99a as well as increased expression of OsHsp18.0-CI and pathogenesis-related genes. Furthermore, we found that OsHsfB4d directly binds to a DNA fragment carrying the only perfect heat shock element (HSE) in the promoter of OsHsp18.0-CI.

Conclusion

Overall, we reveal that OsHsfB4d, a class B Hsf, acts as a positive regulator of OsHsp18.0-CI to mediate BLS and BB resistance in rice.

中文翻译：

OsHsfB4d结合了启动子并调节了OsHsp18.0-CI的表达，使其能抵抗米黄单胞菌。

背景

稻叶枯萎病（BLS）和细菌枯萎病（BB）是稻米黄单胞菌（Xanthomonas oryzae pv）的革兰氏阴性细菌引起的两种主要的破坏性水稻大病。空心稗（XOC）和黄单胞菌PV。稻米（Xoo）。以前，我们鉴定了与国防相关（DR）的基因，该基因编码一种小的热激蛋白OsHsp18.0-CI，可正向调节水稻的BLS和BB抗性。

结果

为了揭示OsHsp18.0 - CI对Xoc和Xoo的调控机制，我们通过转录分析和转基因研究对B类热休克因子（Hsf），OsHsfB4d进行了表征。Xoc菌株RS105或Xoo菌株PXO99a在中华11号（野生型，ZH11）以及过表达OsHsp18.0 - CI的水稻植株中，接种后OsHsfB4d均被上调。OsHsfB4d的瞬时表达可以通过OsHsp18.0-CI启动子激活绿色荧光蛋白（GFP）和荧光素酶（Luc）的表达。水稻过表达OsHsfB4d表现出对RS105和PXO99a增强的抗性以及OsHsp18.0 - CI和发病相关基因的表达增加。此外，我们发现OsHsfB4d直接与在OsHsp18.0-CI启动子中携带唯一完美热激元件（HSE）的DNA片段结合。