A new device that enables the application of controlled mechanical loads to devitalized cortical bone blocks in liquid media mimicking physiological environment is described. In the setup, it is possible to evaluate elimination of calcium from a bone during the exposure to slightly acidic media in the presence or absence of periodic controlled mechanical load. In the test experiments with the fragments of cortical bone, the level of calcium elimination from a bone during the experiment has shown the visible dissimilarity for the loaded bone and the unloaded one. The structural alterations in the apatite of the bone after the experiments in the setup have been studied by X-ray diffraction (XRD), which has shown that the lattice microstrain parameter of the bone mineral noticeably increases in the loaded bone compared with unloaded, while the crystallite size shows only a trend to slight increasing. This simple and easy to reproduce bone-loading setup could be used for in vitro studies of the bone material stability under mechanical loading.

中文翻译：

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一种评估弱酸性介质中动态负载对失活皮质骨生物磷灰石影响的机械装置

描述了一种新设备，它能够将受控机械负载应用于模拟生理环境的液体介质中的失活皮质骨块。在该设置中，可以评估在存在或不存在周期性受控机械负载的情况下暴露于微酸性介质期间钙从骨骼中的消除。在用皮质骨碎片进行的测试实验中，实验过程中从骨骼中消除钙的水平表明加载的骨骼和未加载的骨骼明显不同。通过 X 射线衍射 (XRD) 研究了在装置中的实验后骨骼磷灰石的结构变化，这表明与未加载的骨骼相比，加载的骨骼中骨矿物质的晶格微应变参数显着增加，而微晶尺寸仅呈现略有增加的趋势。这种简单且易于重现的骨负荷设置可用于机械负荷下骨材料稳定性的体外研究。