Cocal virus (COCV) is one of the causative agents of vesicular stomatitis, presenting clinical signs indistinguishable from those caused by foot-and-mouth disease virus (FMDV). Therefore, the differentiation of these two viruses via laboratory diagnosis is essential. The objective of this study was to develop and validate a real-time quantitative PCR (RT-qPCR) protocol for the diagnosis of COCV directly from epithelial samples. The method developed had 97% accuracy at 3950 pfu and a repeatability error of 1.29%. RT-qPCR was able to distinguish COCV from other viruses that cause vesicular diseases, an important factor because seroneutralization may produce cross-reactivity between COCV and vesicular stomatitis Alagoas virus (VSAV). No epithelial sample originating from vesicular disease outbreaks between 2014 and 2018 in Brazil was positive for COCV.

粘膜病毒（COCV）是水疱性口炎的病原体之一，其临床症状与口蹄疫病毒（FMDV）引起的症状没有明显区别。因此，通过实验室诊断区分这两种病毒至关重要。这项研究的目的是开发和验证实时定量PCR（RT-qPCR）协议，以直接从上皮样品中诊断COCV。所开发的方法在3950 pfu时的准确度为97％，重复性误差为1.29％。RT-qPCR能够将COCV与其他引起囊泡疾病的病毒区分开来，这是一个重要因素，因为血清ututralization可能会在COCV和囊泡性口炎Alagoas病毒（VSAV）之间产生交叉反应。