Acinetobacter pittii is an important pathogen causing nosocomial infection worldwide. In this study, a multidrug-resistant A. pittii ABC38 was used as host bacterium to isolate the lytic phage vB_ApiP_XC38. The biological characteristics of vB_ApiP_XC38 were studied and the genome was sequenced and analyzed. vB_ApiP_XC38 belonged to Podoviridae family. The phage had double-stranded genome, which comprised 79,328 bp with 39.58% G+C content displaying very low similarity (< 1% identity) with published genomes of other phages and bacteria. A total of 97 open reading frames (ORFs) were predicted and contained nucleotide metabolism and replication module, structural components module, and lysis module. The ANI, AAI, and phylogenetic analysis indicated that all phages were found distant from vB_ApiP_XC38. Altogether, morphological, genomics, and phylogenetic analysis suggest that vB_ApiP_XC38 is more likely a novel phage of A. pittii.

中文翻译：

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新型噬菌体感染匹氏不动杆菌vB_ApiP_XC38的特征和基因组分析。

匹氏不动杆菌是引起全世界医院感染的重要病原体。在这项研究中，耐多药的匹氏杆菌ABC38被用作宿主细菌，以分离裂解性噬菌体vB_ApiP_XC38。研究了vB_ApiP_XC38的生物学特性，并对基因组进行了测序和分析。vB_ApiP_XC38属于Podoviridae家族。该噬菌体具有双链基因组，其包含79,328 bp，G + C含量为39.58％，与已发表的其他噬菌体和细菌的基因组具有极低的相似性（<1％同一性）。总共预测了97个开放阅读框（ORF），其中包含核苷酸代谢和复制模块，结构成分模块和裂解模块。ANI，AAI和系统发育分析表明，发现所有噬菌体均远离vB_ApiP_XC38。总体而言，形态学，基因组学，