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The characteristics and genome analysis of vB_ApiP_XC38, a novel phage infecting Acinetobacter pittii

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Abstract

Acinetobacter pittii is an important pathogen causing nosocomial infection worldwide. In this study, a multidrug-resistant A. pittii ABC38 was used as host bacterium to isolate the lytic phage vB_ApiP_XC38. The biological characteristics of vB_ApiP_XC38 were studied and the genome was sequenced and analyzed. vB_ApiP_XC38 belonged to Podoviridae family. The phage had double-stranded genome, which comprised 79,328 bp with 39.58% G+C content displaying very low similarity (< 1% identity) with published genomes of other phages and bacteria. A total of 97 open reading frames (ORFs) were predicted and contained nucleotide metabolism and replication module, structural components module, and lysis module. The ANI, AAI, and phylogenetic analysis indicated that all phages were found distant from vB_ApiP_XC38. Altogether, morphological, genomics, and phylogenetic analysis suggest that vB_ApiP_XC38 is more likely a novel phage of A. pittii.

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Acknowledgements

This work was financially supported through grants from the National Natural Science Foundation of China (Nos. 31872505 and U19A2038), the Natural Science Foundation of Jilin Province (Changchun, China; No. 20200201120JC), the Jilin Province Science Foundation for Youths (Changchun, China; No. 20190103106JH), the Achievement Transformation Project of the First Hospital of Jilin University (No. JDYYZH-1902025) the Shanghai Municipal Health Commission Scientific Research Project (No. 20194Y0061) and the Fundamental Research Funds for the Central Universities.

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Author notes

  1. Mengjun Cheng, Man Luo, and Hengyu Xi have contributed equally to this study.

Authors and Affiliations

  1. Shanghai Institute of Phage, Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China

    Mengjun Cheng, Yunze Zhao, Shuai Le, Li-Kuang Chen, Demeng Tan, Nannan Wu, Tongyu Zhu & Jingmin Gu

  2. Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, 130062, China

    Man Luo, Hengyu Xi, Yuan Guan, Wenyu Han & Jingmin Gu

  3. Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, Yangzhou, 225009, China

    Wenyu Han

  4. Department of Microbiology, Army Medical University, Chongqing, 400038, China

    Shuai Le

  5. College of Clinical Medicine, Jilin University, Changchun, 130021, China

    Tianqi Wang

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  1. Mengjun Cheng
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Contributions

NW, TZ, JG: Conceptualization, Funding acquisition and Investigation. MC, ML and HX: Methodology. YZ, DT, L-KC, TW: Data curation, Draft preparation. MC and SL: Writing. SR, YG and WH: Reviewing and Editing.

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Correspondence to Nannan Wu, Tongyu Zhu or Jingmin Gu.

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The authors declare no competing financial and non-financial conflict of interests. The collection of sewage sample had obtained permission of Changchun sewerage system.

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Cheng, M., Luo, M., Xi, H. et al. The characteristics and genome analysis of vB_ApiP_XC38, a novel phage infecting Acinetobacter pittii. Virus Genes 56, 498–507 (2020). https://doi.org/10.1007/s11262-020-01766-0

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