Chrysomelid beetle, Zygogramma bicolorata (Pallister, 1953) (Coleoptera: Chrysomelidae), is an important biocontrol agent for the weed Parthenium hysterophorus. The insect spends its whole life on the same plant, which acts as an essential site for feeding, mating and oviposition. These insects were studied for repeated mating, either to same or different mates, and the results indicated both polyandrous and polygamous mating with same-pair mating preferences. Apart from this, a preliminary bioassay was performed individually for both the sexes to detect the presence of contact pheromones. These chemicals, ranging from C₁₄ to C₃₆ and often known as cuticular hydrocarbons (straight and branched), were further identified by reading their mass spectra. Although the hydrocarbon profiles of males and females resembled each other qualitatively, the compounds showed a significant difference with respect to their relative proportions. The antennal response to some of the corresponding synthetic straight chain alkanes (Pentacosane, Hexatriacontane, Docosane, Tetracosane, Heneicosane, Hexacosane, Octadecane, Eicosane and Tetratriacontane) were also tested using electroantennography (EAG) at four different concentrations (0.1%, 0.01%, 0.001% and 0.0001%). The mean EAG response obtained was observed to be sex dependent for all compounds tested. The antennae of female responded strongly to these compounds with Tetratriacontane eliciting the highest response of – 0.684 mV at the concentration 0.01%. The difference in the responses may be attributed to the differences in the release rates of these compounds and the sensitivity of antennal responses between the sexes.

Chrysomelid甲虫，Zygogramma bicolorata（帕利斯特，1953）（鞘翅目：叶甲科），是用于杂草的重要生物防治剂银胶菊。昆虫的一生都在同一棵植物上，这是昆虫进食，交配和产卵的重要场所。研究了这些昆虫与相同或不同伴侣的重复交配，结果表明，多对和一夫多妻的交配具有相同的配对交配偏好。除此之外，还对男女分别进行了初步的生物测定，以检测接触信息素的存在。这些化学物质，范围从C ₁₄到C ₃₆并通过读取质谱图进一步鉴定通常被称为表皮烃（直链和支链）的碳氢化合物。尽管雄性和雌性的碳氢化合物性质在性质上相似，但这些化合物的相对比例显示出显着差异。还使用电造影（EAG）在四个不同浓度（0.1％，0.01％， 0.001％和0.0001％）。对于所有测试的化合物，观察到获得的平均EAG反应与性别有关。雌性触角对这些化合物的反应强烈，而四金刚烷引起的最高响应为– 0。浓度为0.01％时为684 mV。反应的差异可以归因于这些化合物的释放速率的差异以及两性之间触角反应的敏感性。