There are many studies on the use of lasers as a special mutation-breeding method to select microorganisms and plant seeds. In this study, we screened 72 microalgal mutants from the Chlorella samples irradiated using three types of laser (He-Ne, Nd:YAG and SC). Finally, 2 mutants N2 and S3 from wild Chlorella pyrenoidosa, and 1 mutant, N3 from Chlorella pacifica having high potential for biodiesel applications with high yield and stability, were obtained after comprehensive comparison and systematic screening. The average total lipid productivities of the 3 mutants were 19.90 ± 2.46 mg L⁻¹ day⁻¹ for N2, 29.82 ± 4.45 mg L⁻¹ day⁻¹ for S3 and 20.56 ± 5.69 mg L⁻¹ day⁻¹ for N3, respectively. The 3 mutants were proved they could display higher lipid productivity, lipid content, growth rate and transcriptional expression trends of 7 genes relevant to fatty acid biosynthesis than the wild strain in 10 continuous generation subcultures.

关于使用激光作为一种特殊的突变育种方法来选择微生物和植物种子的研究很多。在这项研究中，我们从使用三种类型的激光（He-Ne，Nd：YAG和SC）照射的小球藻样品中筛选了72个微藻突变体。最后，经过全面比较和系统筛选后，获得了来自野生小球藻的2个突变体N2和S3 ，以及来自小球藻的1个突变体N3，它们具有很高的生物柴油应用潜力，具有高产量和稳定性。3个突变体的平均总脂质生产率对N2为19.90±2.46 mg L ^-1 天^-1，对S3为29.82±4.45 mg L ^-1 天^-1和20.56±5.69 mg LN3分别为^-1 天^-1。这3个突变体被证明与10个连续世代亚培养相比，具有比野生菌株更高的脂生产力，脂含量，生长速率和与脂肪酸生物合成相关的7个基因的转录表达趋势。