Abstract
There are many studies on the use of lasers as a special mutation-breeding method to select microorganisms and plant seeds. In this study, we screened 72 microalgal mutants from the Chlorella samples irradiated using three types of laser (He-Ne, Nd:YAG and SC). Finally, 2 mutants N2 and S3 from wild Chlorella pyrenoidosa, and 1 mutant, N3 from Chlorella pacifica having high potential for biodiesel applications with high yield and stability, were obtained after comprehensive comparison and systematic screening. The average total lipid productivities of the 3 mutants were 19.90 ± 2.46 mg L−1 day−1 for N2, 29.82 ± 4.45 mg L−1 day−1 for S3 and 20.56 ± 5.69 mg L−1 day−1 for N3, respectively. The 3 mutants were proved they could display higher lipid productivity, lipid content, growth rate and transcriptional expression trends of 7 genes relevant to fatty acid biosynthesis than the wild strain in 10 continuous generation subcultures.
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Funding
The present study was supported by National Natural Science Foundation of China (31972815), Major Basic Research Program for Natural Science Foundation of Shandong Province (ZR2019ZD17), Key Research and Development Program of Shandong Province (Food for Special Medical Purpose) (2018YYSP016), Key Research and Development Program of Shandong Province (2017CXGC0309) and the Project of Shandong Province Higher Educational Science and Technology Program (J17KA132).
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In this work, Chunxiao Meng and Zhengquan Gao had completed the experimental design. Kang Wang and Bin Lin had completed data analysis, thesis writing, RNA isolation and real-time qPCR. Zhe Li had completed laser mutagenesis. Haonan Zhang, Huanmin Du and Yanyun Guo had completed microalgal screening, culture and lipid content detection. Fei Xu and Xiao Jiang had completed enzyme activity assay.
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Bin Lin and Kang Wang are co-first authors
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Wang, K., Lin, B., Meng, C. et al. Screening of three Chlorella mutant strains with high lipid production induced by 3 types of lasers. J Appl Phycol 32, 1655–1668 (2020). https://doi.org/10.1007/s10811-020-02112-5
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DOI: https://doi.org/10.1007/s10811-020-02112-5