Clostridium acetobutylicum is a well-known strain for biofuel production. In previous work, it was found that this strain formed biofilm readily during fermentation processes. Biofilm formation could protect cells and enhance productivities under environmental stresses in our previous work. To explore the molecular mechanism of biofilm formation, Spo0A of C. acetobutylicum was selected to investigate its influences on biofilm formation and other physiological performances. When spo0A gene was disrupted, the spo0A mutant could hardly form biofilm. The aggregation and adhesion abilities of the spo0A mutant as well as its swarming motility were dramatically reduced compared to those of wild type strain. Sporulation was also negatively influenced by spo0A disruption, and solvent production was almost undetectable in the spo0A mutant fermentation. Furthermore, proteomic differences between wild type strain and the spo0A mutant were consistent with physiological performances. This is the first study confirming a genetic clue to C. acetobutylicum biofilm and will be valuable for biofilm optimization through genetic engineering in the future.

中文翻译：

---

Spo0A 对丙酮丁醇梭菌的影响，重点是生物膜形成

丙酮丁醇梭菌是众所周知的生物燃料生产菌株。在之前的工作中，发现该菌株在发酵过程中很容易形成生物膜。在我们之前的工作中，生物膜的形成可以在环境压力下保护细胞并提高生产力。为了探索生物膜形成的分子机制，选择了丙酮丁醇梭菌的 Spo0A 来研究其对生物膜形成和其他生理性能的影响。当 spo0A 基因被破坏时，spo0A 突变体几乎不能形成生物膜。与野生型菌株相比，spo0A 突变体的聚集和粘附能力及其蜂群运动能力显着降低。孢子形成也受到 spo0A 破坏的负面影响，并且在 spo0A 突变体发酵中几乎检测不到溶剂的产生。此外，野生型菌株和 spo0A 突变体之间的蛋白质组学差异与生理表现一致。这是第一项确认丙酮丁醇梭菌生物膜遗传线索的研究，对于未来通过基因工程优化生物膜具有重要价值。