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Misidentification of Cutibacterium namnetense as Cutibacterium acnes among clinical isolates by MALDI-TOF VitekMS: usefulness of gyrB sequencing and new player in bone infections.
European Journal of Clinical Microbiology & Infectious Diseases ( IF 3.7 ) Pub Date : 2020-05-07 , DOI: 10.1007/s10096-020-03873-0
L Ruffier d'Epenoux 1 , N Arshad 1 , P Bémer 1, 2 , M-E Juvin 1 , G Le Gargasson 1 , A Guillouzouic 1 , S Corvec 1, 2, 3, 4, 5
Affiliation  

The taxonomy modification of Propionibacterium sp. with the description of new species, especially Cutibacterium namnetense, raises the question of species distribution in routine clinical samples. We performed a retrospective study during 3 years before the implementation of MALDI-TOF. Two hundred sixty-nine isolates were included in the study. MALDI-TOF identification, 16S rRNA, and new developed gyrB partial sequencings were performed. The most representative species was C. acnes in 88% of the cases, regardless of the origin of the clinical sample. Eventually, we identified three C. namnetense strains, representing a 1.1% prevalence over the period of time, including two bone infections. MALDI-TOF databases should be regularly updated to incorporate new species. gyrB sequencing constitutes a both easy and relevant method to identify Cutibacterium sp. especially C. namnetense, a new player in bone infections.



中文翻译:

MALDI-TOF VitekMS在临床分离株中将南分枝杆菌误认为是痤疮痤疮杆菌:gyrB测序的有用性和新参与者在骨感染中的作用。

丙酸杆菌属的分类学修饰。通过描述新物种,尤其是南分枝杆菌,提出了常规临床样品中物种分布的问题。在实施MALDI-TOF之前的3年中,我们进行了一项回顾性研究。该研究包括269个分离株。进行MALDI-TOF鉴定,16S rRNA和新开发的gyrB部分测序。不管临床样品的来源如何,最有代表性的物种是88%的痤疮丙酸杆菌。最终,我们确定了三个C. namnetense毒株,在一段时间内占1.1%的患病率,其中包括两次骨感染。MALDI-TOF数据库应定期更新以纳入新物种。gyrB测序构成了一种鉴定皮肤杆菌的简便而相关的方法。特别是C. namnetense,这是骨骼感染的新参与者。

更新日期:2020-05-07
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