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Misidentification of Cutibacterium namnetense as Cutibacterium acnes among clinical isolates by MALDI-TOF VitekMS: usefulness of gyrB sequencing and new player in bone infections

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Abstract

The taxonomy modification of Propionibacterium sp. with the description of new species, especially Cutibacterium namnetense, raises the question of species distribution in routine clinical samples. We performed a retrospective study during 3 years before the implementation of MALDI-TOF. Two hundred sixty-nine isolates were included in the study. MALDI-TOF identification, 16S rRNA, and new developed gyrB partial sequencings were performed. The most representative species was C. acnes in 88% of the cases, regardless of the origin of the clinical sample. Eventually, we identified three C. namnetense strains, representing a 1.1% prevalence over the period of time, including two bone infections. MALDI-TOF databases should be regularly updated to incorporate new species. gyrB sequencing constitutes a both easy and relevant method to identify Cutibacterium sp. especially C. namnetense, a new player in bone infections.

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Acknowledgments

We are grateful for the lab technicians for their implication in Cutibacterium species recovering. We would like to thank Andrej Trampuz and Brigitte Dréno for their helpful discussions.

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Authors and Affiliations

  1. Service de Bactériologie-Hygiène Hospitalière, CHU Nantes, Nantes Université, Nantes, France

    L. Ruffier d’Epenoux, N. Arshad, P. Bémer, M-E. Juvin, G. Le Gargasson, A. Guillouzouic & S. Corvec

  2. Nantes study group member of CRIOGO (Centre de Référence des Infections Ostéo-articulaires du Grand Ouest), Nantes, France

    P. Bémer & S. Corvec

  3. CRCINA, INSERM U1232, Nantes Université, Nantes, France

    S. Corvec

  4. ESCMID Study Group for Implant-Associated Infections (ESGIAI) member, Basel, Switzerland

    S. Corvec

  5. Institut de Biologie des Hôpitaux de Nantes, Service de Bactériologie-Hygiène, CHU de Nantes, 9 quai Moncousu, 44093, Nantes Cedex 01, France

    S. Corvec

Authors
  1. L. Ruffier d’Epenoux
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  2. N. Arshad
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  3. P. Bémer
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  4. M-E. Juvin
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  5. G. Le Gargasson
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  6. A. Guillouzouic
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  7. S. Corvec
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Correspondence to S. Corvec.

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The authors declare that they have no conflict of interest.

Ethical approval

According to the French and European legislations, the use of anonymous data does not need an approval of the ethics committee. This study has been recorded in the Nantes Hospital by the local’s Data Protection Officer under reference TS005.BIO.AP.2019_1.

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Ruffier d’Epenoux, L., Arshad, N., Bémer, P. et al. Misidentification of Cutibacterium namnetense as Cutibacterium acnes among clinical isolates by MALDI-TOF VitekMS: usefulness of gyrB sequencing and new player in bone infections. Eur J Clin Microbiol Infect Dis 39, 1605–1610 (2020). https://doi.org/10.1007/s10096-020-03873-0

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  • DOI: https://doi.org/10.1007/s10096-020-03873-0

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