Nitrate-denitrifying anaerobic methane oxidation (Nitrate-DAMO) process is a novel bioprocess which couples anaerobic oxidation of methane (AOM) with nitrite/nitrate reduction. In this study, one Nitrate-DAMO culture contains both DAMO bacteria (62.2%) and DAMO archaea (26.5%). The environmental conditions, temperature, pH, and dissolved oxygen were found to affect Nitrate-DAMO activity significantly. When temperature was below 35 °C, Nitrate-DAMO reaction rate dropped as temperature decreased, and when temperature was above 35 °C, Nitrate-DAMO reaction rate decreased as temperature rose; Nitrate-DAMO reaction rate reached maximum at 35 °C. When pH was below 6.5, Nitrate-DAMO reaction rate decreased with the decrease of pH, and when pH was above 7.5, Nitrate-DAMO reaction rate decreased as pH rose; the maximum Nitrate-DAMO reaction rate appeared at both pH 6.5 and 7.5. Nitrate-DAMO activity was inversely proportional to dissolved oxygen concentration, which meant the existence of dissolved oxygen inhibited Nitrate-DAMO process.

硝酸盐反硝化厌氧甲烷氧化（Nitrate-DAMO）工艺是一种新型的生物工艺，将甲烷的厌氧氧化（AOM）与亚硝酸盐/硝酸盐还原相结合。在这项研究中，一种硝酸盐-DAMO培养物同时含有DAMO细菌（62.2％）和DAMO古细菌（26.5％）。发现环境条件，温度，pH和溶解氧会显着影响硝酸盐-DAMO的活性。当温度低于35℃时，硝酸盐-DAMO反应速率随温度降低而降低；而当温度高于35℃时，硝酸盐-DAMO反应速率随温度升高而降低。硝酸盐-DAMO反应速率在35°C时达到最大值。当pH值低于6.5时，硝酸盐-DAMO反应速率随pH值的降低而降低；当pH值高于7.5时，硝酸盐-DAMO反应速率随pH值的升高而降低。在pH 6.5和7.5时出现最大的硝酸盐-DAMO反应速率。硝酸盐-DAMO活性与溶解氧浓度成反比，这意味着溶解氧的存在抑制了硝酸盐-DAMO过程。