The current research was aimed to carry out the synthesis and characterization of silver nanoparticles (AgNPs) from the Acacia nilotica leaf extract mediated compound pyrogallol (py). The py-AgNPs were evaluated for in vitro antioxidant, antimicrobial, and biofilm activities against Helicobacter pylori (H. pylori). Further, the apoptotic induction of human gastric adenocarcenoma (AGS) cells was also investigated. Characterization of py-AgNPs was evaluated by using Fourier transform infrared spectroscopy (FT-IR), High-resolution transmission electron microscopy (HR-TEM), Scanning electron microscopy (SEM), Zeta potential, dynamic light scattering (DLS), X-ray diffraction (XRD) and UV–visible spectrometry. The average size of py-AgNPs was 22.68–55.16 nm in range, confirmed by HR-TEM. The clear H. pylori biofilm and bacterial inhibition were observed by increasing the concentration. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on AGS cells were revealed that the increasing py-AgNPs level, significantly decreases the cell viability by increased apoptosis. The cell cycle arrest was observed. These results indicate that py-AgNPs have a vital function as an anti-H. pylori and anti-gastric cancer inhibitor and could be further used in the development of nanomedicines to counter lethal diseases.

当前的研究旨在从相思叶提取物介导的化合物邻苯三酚（py）中合成银纳米颗粒（AgNPs ）。评估py-AgNPs对幽门螺杆菌（H. pylori）的体外抗氧化，抗菌和生物膜活性）。此外，还研究了人胃腺癌（AGS）细胞的凋亡诱导。通过使用傅立叶变换红外光谱（FT-IR），高分辨率透射电子显微镜（HR-TEM），扫描电子显微镜（SEM），Zeta电位，动态光散射（DLS），X-射线衍射（XRD）和紫外可见光谱。HR-TEM证实py-AgNPs的平均大小在22.68–55.16 nm范围内。透明幽门螺杆菌通过增加浓度观察到生物膜和细菌抑制。在AGS细胞上进行的3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）分析表明，增加的py-AgNPs水平会通过增加凋亡来显着降低细胞活力。观察到细胞周期停滞。这些结果表明，py-AgNP具有作为抗幽门螺杆菌和抗胃癌抑制剂的重要功能，并且可以进一步用于开发用于对抗致死性疾病的纳米药物。