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Synthesis and Characterization of Pyrogallol Capped Silver Nanoparticles and Evaluation of Their In Vitro Anti-Bacterial, Anti-cancer Profile Against AGS Cells

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Abstract

The current research was aimed to carry out the synthesis and characterization of silver nanoparticles (AgNPs) from the Acacia nilotica leaf extract mediated compound pyrogallol (py). The py-AgNPs were evaluated for in vitro antioxidant, antimicrobial, and biofilm activities against Helicobacter pylori (H. pylori). Further, the apoptotic induction of human gastric adenocarcenoma (AGS) cells was also investigated. Characterization of py-AgNPs was evaluated by using Fourier transform infrared spectroscopy (FT-IR), High-resolution transmission electron microscopy (HR-TEM), Scanning electron microscopy (SEM), Zeta potential, dynamic light scattering (DLS), X-ray diffraction (XRD) and UV–visible spectrometry. The average size of py-AgNPs was 22.68–55.16 nm in range, confirmed by HR-TEM. The clear H. pylori biofilm and bacterial inhibition were observed by increasing the concentration. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on AGS cells were revealed that the increasing py-AgNPs level, significantly decreases the cell viability by increased apoptosis. The cell cycle arrest was observed. These results indicate that py-AgNPs have a vital function as an anti-H. pylori and anti-gastric cancer inhibitor and could be further used in the development of nanomedicines to counter lethal diseases.

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Acknowledgment

The authors would like to thank the Taiwan Experience Education Program, National Pingtung University, Taiwan, for providing laboratory facilities and financial support.

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Authors and Affiliations

  1. Department of Zoology, School of Life Sciences, Periyar University, Salem, Tamil Nadu, 636 011, India

    Gattu Sampath & Nagarajan Kayalvizhi

  2. Functional Genomics Laboratory, Department of Biological Science and Technology, National Pingtung University of Science and Technology, Neipu, Pingtung, 91201, Taiwan

    Douglas J. H. Shyu

  3. School of Life Sciences, Central University of Tamil Nadu, Thiruvarur, Tamil Nadu, 610005, India

    Neelamegam Rameshkumar

  4. Madurai Kamaraj University, Madurai, Tamil Nadu, 625 021, India

    Muthukalingan Krishnan

  5. Department of Environmental Science, School of Life Sciences, Periyar University, Salem, Tamil Nadu, 636 011, India

    Palaniappan Sivasankar

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  1. Gattu Sampath
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Correspondence to Nagarajan Kayalvizhi.

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Sampath, G., Shyu, D.J.H., Rameshkumar, N. et al. Synthesis and Characterization of Pyrogallol Capped Silver Nanoparticles and Evaluation of Their In Vitro Anti-Bacterial, Anti-cancer Profile Against AGS Cells. J Clust Sci 32, 549–557 (2021). https://doi.org/10.1007/s10876-020-01813-8

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