A rapid and sensitive spectrophotometric method has been developed for the quantitative analysis of three antiviral, anti-parkinsonism drugs, namely, amantadine (AMA), memantine (MET), and rimantadine (RIM). The method is based on the usage of 1,3-indandione (IDO) as a chromogenic reagent to form charge transfer complexes with the studied drugs and produce colored reaction products with an absorbance maximum at 522 nm, allowing quantitative analysis of these drugs. In addition, the study was validated according to the official guidelines that permits usage in quality control laboratories. Many factors (reagent volume, diluting solvent, temperature, reaction and stability time) influencing the reactions were studied and optimized. The results showed that this method is able to detect AMA, MET, or RIM over a linear range between 10–140 μg/mL with high selectivity and robustness. Furthermore, the study results were applied to analyze the drugs in their pharmaceutical preparations with acceptable accuracy and precision.

中文翻译：

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快速简便的分光光度法测定抗病毒和抗帕金森病药物

已经开发了一种快速灵敏的分光光度法，用于定量分析三种抗病毒，抗帕金森病药物，即金刚烷胺（AMA），美金刚（MET）和金刚乙胺（RIM）。该方法基于使用1,3-茚满二酮（IDO）作为生色试剂，与所研究的药物形成电荷转移络合物，并产生有色反应产物，最大吸收度为522 nm，从而可以对这些药物进行定量分析。此外，该研究还根据允许在质量控制实验室中使用的官方指南进行了验证。研究和优化了影响反应的许多因素（试剂量，稀释溶剂，温度，反应和稳定时间）。结果表明该方法能够检测AMA，MET，或RIM在10–140μg/ mL的线性范围内，具有高选择性和耐用性。此外，研究结果被用于以可接受的准确性和精确度分析药物制剂中的药物。