This study aimed to investigate the effect of fractionated seminal plasma on characteristics of common carp Cyprinus carpio cryopreserved sperm. Nanosep® centrifugal devices yielded four seminal plasma fractions with different total protein content ranging in molecular weight from less than 17 to almost 74 kDa. Each protein fraction was added to semen extender medium prior to freezing. Spermatozoon motility characteristics and DNA integrity were analyzed in supplemented and non-supplemented cryopreserved samples. The cryopreservation process strongly affected the swim-up sperm quality. Treatment with fractions 1, 2, 3, and 4 was associated with significantly higher spermatozoon motility rate and curvilinear velocity than seen in extender only, with highest values obtained with fraction 4 (78.21 ± 2.41% and 168.05 ± 4.46 μm/s, respectively). Significantly less DNA damage, expressed as percent tail DNA (12.23 ± 1.27) and olive tail moment (0.68 ± 0.12), was recorded in fraction 4. The findings indicated that addition of fractionated seminal plasma to cryopreservation medium can preserve the quality of common carp sperm. The protective effect of each fraction varied, suggesting the presence of distinct components exerting different effects on cryopreserved sperm function.

中文翻译：

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精浆血浆级分可以在冷冻保存过程中保护鲤鱼（Cyprinus carpio）的精子。

本研究旨在探讨精浆分离对鲤鱼冷冻保存的精子特性的影响。Nanosep®离心设备产生了四个精浆级分，其总蛋白质含量不同，分子量范围从小于17到几乎74 kDa。在冷冻之前，将每种蛋白质级分添加到精液增量剂培养基中。在补充和未补充冷冻保存的样品中分析了精子运动特征和DNA完整性。冷冻保存过程强烈影响精子繁殖质量。分数1、2、3和4的处理与仅在补充剂中观察到的精子运动率和曲线速度显着相关，分数4可获得最高值（分别为78.21±2.41％和168.05±4.46μm/ s）。 。在级分4中记录到的DNA损伤明显更少，以尾部DNA百分比（12.23±1.27）和橄榄尾部矩（0.68±0.12）表示。研究结果表明，在冷冻保存培养基中添加分馏的精浆可以保留鲤鱼的品质。精子。每个部分的保护作用各不相同，表明存在不同成分对冷冻保存的精子功能产生不同影响。