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Seminal plasma fractions can protect common carp (Cyprinus carpio) sperm during cryopreservation

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Abstract

This study aimed to investigate the effect of fractionated seminal plasma on characteristics of common carp Cyprinus carpio cryopreserved sperm. Nanosep® centrifugal devices yielded four seminal plasma fractions with different total protein content ranging in molecular weight from less than 17 to almost 74 kDa. Each protein fraction was added to semen extender medium prior to freezing. Spermatozoon motility characteristics and DNA integrity were analyzed in supplemented and non-supplemented cryopreserved samples. The cryopreservation process strongly affected the swim-up sperm quality. Treatment with fractions 1, 2, 3, and 4 was associated with significantly higher spermatozoon motility rate and curvilinear velocity than seen in extender only, with highest values obtained with fraction 4 (78.21 ± 2.41% and 168.05 ± 4.46 μm/s, respectively). Significantly less DNA damage, expressed as percent tail DNA (12.23 ± 1.27) and olive tail moment (0.68 ± 0.12), was recorded in fraction 4. The findings indicated that addition of fractionated seminal plasma to cryopreservation medium can preserve the quality of common carp sperm. The protective effect of each fraction varied, suggesting the presence of distinct components exerting different effects on cryopreserved sperm function.

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Funding

This study is supported by the Ministry of Education, Youth and Sports of the Czech Republic, projects CENAKVA (LM2018099), and Biodiversity (CZ.02.1.01/0.0/0.0/16_025/0007370) as well as by the CAS President’s International Fellowship for Postdoctoral Researchers (2017 PB0060), National Natural Science Foundation of China (21676286), and Primary Research Development Plan of Shandong Province (2016GSF121006). The Lucidus Consultancy, UK is gratefully acknowledged for the English correction.

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Authors and Affiliations

  1. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25, Vodňany, Czech Republic

    Anna Shaliutina-Kolešová

  2. Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, China

    Anna Shaliutina-Kolešová, Mo Xian & Rui Nian

  3. Department of Physical Chemistry, University of Chemistry and Technology, Prague, Technická 5, 16628, Prague 6, Czech Republic

    Saeed Ashtiani

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  1. Anna Shaliutina-Kolešová
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  2. Saeed Ashtiani
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  3. Mo Xian
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  4. Rui Nian
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Correspondence to Anna Shaliutina-Kolešová or Rui Nian.

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Shaliutina-Kolešová, A., Ashtiani, S., Xian, M. et al. Seminal plasma fractions can protect common carp (Cyprinus carpio) sperm during cryopreservation. Fish Physiol Biochem 46, 1461–1468 (2020). https://doi.org/10.1007/s10695-020-00805-9

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  • DOI: https://doi.org/10.1007/s10695-020-00805-9

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