To express a TAT-PBX1 fusion protein using a prokaryotic expression system and to explore potential effects of TAT-PBX1 in the proliferation and senescence of human hair follicle-derived mesenchymal stem cells. The TAT-PBX1 fusion was produced in inclusion bodies and heterogenously expressed in Rosetta (DE3) cells. Immunofluorescence staining showed that TAT-PBX1 fusion proteins were internalized by human hair follicle-derived mesenchymal stem cells. The growth rate of cells was increased after treatment with more than 5.0 μg/mL of TAT-PBX1. The rate of senescence-associated β-galactosidase positive cells was reduced in the 10.0 μg/mL TAT-PBX1 group (28%) than the 0 μg/mL control group (60%). Cells treated with the TAT-PBX1 fusion protein showed higher expression of p-AKT (1.22-fold that of the control), which indicates that TAT-PBX1 activated AKT pathway after cellular uptake. The TAT-PBX1 fusion protein increased the proliferation of hair follicle mesenchymal stem cells and delayed their senescence by activating the AKT pathway following internalization by cells.

中文翻译：

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TAT-PBX1融合蛋白的内化显着增强人毛囊源性间充质干细胞的增殖并延缓其衰老


利用原核表达系统表达TAT-PBX1融合蛋白，并探讨TAT-PBX1对人毛囊间充质干细胞增殖和衰老的潜在影响。 TAT-PBX1 融合体在包涵体中产生，并在 Rosetta (DE3) 细胞中异质表达。免疫荧光染色显示 TAT-PBX1 融合蛋白被人毛囊来源的间充质干细胞内化。用超过5.0 μg/mL的TAT-PBX1处理后细胞的生长速率增加。 10.0 μg/mL TAT-PBX1 组 (28%) 的衰老相关 β-半乳糖苷酶阳性细胞率比 0 μg/mL 对照组 (60%) 降低。用TAT-PBX1融合蛋白处理的细胞显示出更高的p-AKT表达（是对照的1.22倍），这表明TAT-PBX1在细胞摄取后激活了AKT通路。 TAT-PBX1融合蛋白通过细胞内化后激活AKT途径来增加毛囊间充质干细胞的增殖并延缓其衰老。