The enzymatic hydrolysis of sugarcane bagasse cellulose (SBC) was performed after acid/peroxide-alkali (APA) pretreatment, soda/AQ pulping, and bleaching steps for producing glucose. Cellic® CTec3 (CT) and Celluclast®1.5L (CL) complexes were evaluated in both individual and mixed systems for observing their influence in cellulose conversion. Deconvolution of XRD patterns and SEM images proved the low crystallinity and high accessibility of SBC, thus being able to provide significantly higher glucose yield than the commercial cellulose with predominant Iβ polymorphism. The analysis of variance (ANOVA) and the response surface methodology (RSM), applied to a full factorial 2⁴ design of experiments built for assessing the activity of CT and CL enzymes together in the hydrolytic system, were accurate in describing that the feedstock (SBC or commercial cellulose) and the treatment of cellulose are the factors with major effect on the saccharification efficiency (F = 744.12, p < 0.0001), thus predicting 53.71 g/L maximum glucose yield for the experimental conditions studied (near to the maximum yield reached, 55.77%) when using SBC, more than double the conversion reached for commercial cellulose, and showing the cooperative work of both complexes together for converting cellulose.

Graphical abstract

在酸/过氧化物-碱（APA）预处理，苏打/ AQ制浆和用于生产葡萄糖的漂白步骤之后，进行了甘蔗渣蔗糖纤维素（SBC）的酶水解。在单个系统和混合系统中都对Cellic®CTec3（CT）和Celluclast®1.5L（CL）复合物进行了评估，以观察它们对纤维素转化的影响。XRD图谱和SEM图像的反卷积证明了SBC的低结晶度和高可及性，因此与具有主要Iβ多态性的商业纤维素相比，能够提供明显更高的葡萄糖产率。方差分析（ANOVA）和响应面方法（RSM），适用于完整阶乘2 ⁴设计用于评估水解系统中CT和CL酶的活性的实验设计，准确地描述了原料（SBC或商业纤维素）和纤维素的处理是对糖化效率产生重大影响的因素（F  = 744.12，p  <0.0001），从而预测在使用SBC时，所研究的实验条件下最大葡萄糖产量为53.71 g / L（接近最大产量，达到55.77％），是商业纤维素转化率的两倍以上，并显示出协同作用两种配合物共同作用来转化纤维素。

图形概要