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Response surface methodology (RSM) for assessing the effects of pretreatment, feedstock, and enzyme complex association on cellulose hydrolysis

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Abstract

The enzymatic hydrolysis of sugarcane bagasse cellulose (SBC) was performed after acid/peroxide-alkali (APA) pretreatment, soda/AQ pulping, and bleaching steps for producing glucose. Cellic® CTec3 (CT) and Celluclast®1.5L (CL) complexes were evaluated in both individual and mixed systems for observing their influence in cellulose conversion. Deconvolution of XRD patterns and SEM images proved the low crystallinity and high accessibility of SBC, thus being able to provide significantly higher glucose yield than the commercial cellulose with predominant Iβ polymorphism. The analysis of variance (ANOVA) and the response surface methodology (RSM), applied to a full factorial 24 design of experiments built for assessing the activity of CT and CL enzymes together in the hydrolytic system, were accurate in describing that the feedstock (SBC or commercial cellulose) and the treatment of cellulose are the factors with major effect on the saccharification efficiency (F = 744.12, p < 0.0001), thus predicting 53.71 g/L maximum glucose yield for the experimental conditions studied (near to the maximum yield reached, 55.77%) when using SBC, more than double the conversion reached for commercial cellulose, and showing the cooperative work of both complexes together for converting cellulose.

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Acknowledgments

The authors acknowledge the support provided by the Post-Graduate Programs PPGE/UNIFACS, PPGQ/UFRN, PPEQ/UFBA, and PPGEQ/UFSCar.

Funding

This study was financed by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES – Brazil) - Finance Code 001 and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPQ – Brazil).

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Authors and Affiliations

  1. Energy Post-Graduate Program, Salvador University (UNIFACS), Salvador, BA, 40140-110, Brazil

    Leila M. A. Campos

  2. Energetic Technologies Laboratory - LABTEN, Chemistry Institute, Federal University of Rio Grande do Norte (UFRN), Natal, RN, 59078-970, Brazil

    Heloise O. M. A. Moura & Luciene S. de Carvalho

  3. Chemical Engineering Department, Federal University of São Carlos, São Carlos, SP, 13565-905, Brazil

    Antonio J. G. Cruz

  4. Chemical Engineering Department, Federal University of Bahia (UFBA), Salvador, BA, 40210-630, Brazil

    Samira M. N. Assumpção & Luiz A. M. Pontes

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  1. Leila M. A. Campos
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  2. Heloise O. M. A. Moura
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Correspondence to Leila M. A. Campos or Luciene S. de Carvalho.

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Campos, L.M.A., Moura, H.O.M.A., Cruz, A.J.G. et al. Response surface methodology (RSM) for assessing the effects of pretreatment, feedstock, and enzyme complex association on cellulose hydrolysis. Biomass Conv. Bioref. 12, 2811–2822 (2022). https://doi.org/10.1007/s13399-020-00756-4

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