PURPOSE OF REVIEW Emerging single-cell RNA sequencing technologies hold great promises to boost our understanding of the heterogeneity and molecular regulation of diverse cell phenotypes during organ development. In this review, we aimed at summarizing recent advances in employing single-cell transcriptomic analysis to depict the landscape of embryonic heart development, in particular, focusing on cardiac progenitor (CP) differentiation. RECENT FINDINGS Recent studies unbiasedly cataloged and characterized cardiac cell types in the spatial and temporal resolution during early heart development. Pseudo-time analysis revealed a temporal continuum of the differentiation progress from embryonic day (E) 6.5 to E9.5, implicating early cardiac lineage restriction during mouse gastrulation. First and second heart field (FHF and SHF) CPs adopted different differentiation strategies and underwent distinct transcriptional regulation. Collectively, the comprehensive molecular atlases yield a rich resource for identification of the key cardiac regulators and signaling molecules within the key cardiac gene regulatory network (GRN) governing cardiac cell fate determinations. This review offers insights into the exquisite process and its regulation of CP differentiation at single-cell resolution. As single-cell technologies continuously grow and evolve, computational integration of multimodal single-cell data with well-designed experimental validation promises to further delineate molecular basis in deploying cardiac progenitors of distinct sources with anatomical information.

中文翻译：

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心脏祖细胞分化的单细胞转录组学分析。

审查的目的新兴的单细胞RNA测序技术具有广阔的前景，可以增进我们对器官发育过程中多种细胞表型的异质性和分子调控的了解。在这篇综述中，我们旨在总结利用单细胞转录组学分析来描述胚胎心脏发育情况的最新进展，特别是关注心脏祖细胞（CP）的分化。最近的发现最近的研究在心脏早期发育过程中，在时空分辨率上对心脏细胞类型进行了分类和表征。伪时间分析揭示了从胚胎天（E）6.5到E9.5的分化过程在时间上的连续性，这暗示了小鼠胃泌乳过程中早期心脏谱系的限制。第一和第二心脏领域（FHF和SHF）CP采取了不同的分化策略，并经历了不同的转录调控。总的来说，全面的分子图谱提供了丰富的资源，可用于识别主要的心脏调节剂和决定心脏命运的关键心脏基因调节网络（GRN）中的信号分子。这篇综述提供了对精巧过程及其在单细胞分辨率下对CP分化的调控的见解。随着单细胞技术的不断发展和发展，多模式单细胞数据与精心设计的实验验证的计算集成有望进一步勾勒出分子基础，以利用解剖信息来部署不同来源的心脏祖细胞。