Probiotic bacteria are known to exert a wide range of anticancer activities on their animal hosts. In the present study, the anticancer effect of a cocktail of several potential probiotic Lactobacillus species (potential probiotic L.C) was investigated in vitro and in vivo. MTT and Flow cytometry tests results showed that administration of live potential probiotic L.C significantly decreased the HT-29 and CT-26 cells proliferation and induced late apoptotis in a time-dependent manner. In addition, quantitative real-time polymerase chain reaction (qPCR) results showed that exposure of potential probiotic L.C to both HT-29 and CT-26 cells during the incubation times resulted in the upregulation (apc and CSNK1ε for HT-29, CSNK1ε and gsk3β for CT-26) and downregulation (CTNNB1, CCND1, pygo2, axin2 and id2) of the Wnt/β- catenin pathway-related genes in a time-dependent manner. The significance of in vitro anticancer effect of potential probiotic L.C was further confirmed in an experimental tumor model. Data from the murine model of colorectal cancer (CRC) induced by Azoxymethane (AOM) and Dextran Sulfate Sodium (DSS) showed significantly alleviated inflammation and tumor development in AOM/DSS/L.C-injected mice compared to the AOM/DSS-injected mice. Tumor growth inhibition was accompanied by potential probiotic L.C-driven upregulation and downregulation of the Wnt/β-catenin pathway-related genes, similar to the in vitro results. These results showed that potential probiotic L.C inhibited the tumor growth, and that its anticancer activity was at least partially mediated through suppressing the Wnt/β-catenin pathway. Overall, the present study suggested that this probiotic could be used clinically as a supplement for CRC prevention and treatment.

中文翻译：

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乳杆菌物种通过调节Wnt /β-catenin信号通路对大肠癌进展的抑制作用。

已知益生菌对动物宿主具有广泛的抗癌活性。在本研究中，体外和体内研究了几种潜在益生菌乳酸菌（潜在益生菌LC）混合物的抗癌作用。MTT和流式细胞仪测试结果表明，使用活的潜在益生菌LC可以显着降低HT-29和CT-26细胞的增殖，并以时间依赖性方式诱导晚期凋亡。此外，实时定量聚合酶链反应（qPCR）定量结果表明，潜伏期的益生菌LC在孵育时间内同时暴露于HT-29和CT-26细胞会导致上调（HT-29，apc和CSNK1ε以及HT-29，CSNK1ε和gsk3β（用于CT-26）和下调（CTNNB1，CCND1，pygo2，Wnt /β-catenin途径相关基因的axin2和id2）呈时间依赖性。在实验肿瘤模型中进一步证实了潜在益生菌LC的体外抗癌作用的重要性。由Azoxymethane（AOM）和右旋糖酐硫酸钠（DSS）诱导的大肠癌（CRC）鼠模型的数据显示，与AOM / DSS / LC注射的小鼠相比，注射AOM / DSS / LC的小鼠的炎症和肿瘤发展得到了显着缓解。与体外结果相似，肿瘤生长抑制伴随着益生菌LC驱动的Wnt /β-catenin途径相关基因的上调和下调。这些结果表明潜在的益生菌LC抑制了肿瘤的生长，并且其抗癌活性至少部分通过抑制Wnt /β-catenin途径介导。总体，