Effect of sinusoidal vibration on the activity of some antioxidative enzymes, pigments, membrane stability, and total phenolic was investigated in callus tissues of Matricaria chamomilla L. Two calli sources were initiated from leaf and root explants, and different frequencies of sinusoidal vibration (0, 5, 10, 15, 50, and 100 Hz) for 3 min were applied to stimulate callus tissues of M. chamomilla. According to the obtained findings in two calli of Matricaria chamomilla, enhancement of growth by vibration was observed at 15 and 50 Hz. Improvement of growth by vibration can be explained by induction in protein, proline total phenol contents, superoxide dismutase, peroxidase, catalase and ascorbate peroxidase activities, reduction in malondialdehyde, and hydrogen peroxide levels. Carotenoid significantly augmented at 15 and 50 Hz frequencies. The electrophoresis profile showed different isoform patterns, and the intensity of bands was promoted by vibration. Moreover, a new superoxide dismutase band appeared at 15 and 50 Hz frequencies in leaf calli. These results may provide insight into antioxidant defense mechanisms of callus tissue to vibration.

研究了正弦振动对洋甘菊愈伤组织中某些抗氧化酶，色素，膜稳定性和总酚的活性的影响。叶和根外植体引发了两个愈伤组织来源，并且正弦振动的频率不同（0， 5，10，15，50，和100Hz）3分钟施加刺激的愈伤组织M.菊。根据获得的发现，在两个母菊洋甘菊的愈伤组织中，在15和50 Hz观察到振动引起的生长增强。振动引起的生长改善可以通过诱导蛋白质，脯氨酸总酚含量，超氧化物歧化酶，过氧化物酶，过氧化氢酶和抗坏血酸过氧化物酶活性，丙二醛减少和过氧化氢来解释。类胡萝卜素以15和50 Hz的频率显着增加。电泳图谱显示出不同的同工型模式，并且通过振动促进了条带的强度。此外，在叶片愈伤组织中以15和50 Hz的频率出现了一个新的超氧化物歧化酶带。这些结果可提供对愈伤组织振动的抗氧化防御机制的见解。