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Surfactant and oil formulations for monodisperse droplet emulsion PCR.
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-05-15 , DOI: 10.1039/d0lc00052c Fangli Zhang 1 , Peiyu Liao , Yue Sun , Zitian Chen , Yuhong Pang , Yanyi Huang
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-05-15 , DOI: 10.1039/d0lc00052c Fangli Zhang 1 , Peiyu Liao , Yue Sun , Zitian Chen , Yuhong Pang , Yanyi Huang
Affiliation
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Emulsion PCR has become a popular and widely applied method in biological research and clinical diagnostics to provide evenly amplified products and perform highly quantitative counting of target sequences. However, there is still a lack of information to support further development of appropriate water-in-oil emulsion formulations, which need to be both thermally and mechanically stable for digital amplification reactions. Here, we present a systematic survey of the oil and surfactant components of stable monodisperse w/o emulsions suitable for use with our previously developed micro-capillary array (MiCA)-based centrifugal emulsion generation method. Our findings show that a binary formula consisting of isopropyl palmitate and a silicone copolymer demonstrated the best performance, and provided a general guideline for the development of emulsion systems for digital PCR and emulsion amplification applications.
中文翻译：
用于单分散液滴乳液PCR的表面活性剂和油配方。
乳液PCR已成为生物学研究和临床诊断中一种流行且广泛应用的方法，以提供均匀扩增的产物并对靶序列进行高度定量的计数。然而，仍然缺乏信息来支持进一步开发合适的油包水乳液制剂，对于数字扩增反应，该制剂必须在热和机械上均稳定。在这里，我们对适用于我们以前开发的基于微毛细管阵列（MiCA）的离心乳液生成方法的稳定单分散w / o乳液的油和表面活性剂成分进行了系统的调查。我们的发现表明，由棕榈酸异丙酯和有机硅共聚物组成的二元配方具有最佳性能，
更新日期：2020-06-30
中文翻译：
用于单分散液滴乳液PCR的表面活性剂和油配方。
乳液PCR已成为生物学研究和临床诊断中一种流行且广泛应用的方法，以提供均匀扩增的产物并对靶序列进行高度定量的计数。然而，仍然缺乏信息来支持进一步开发合适的油包水乳液制剂，对于数字扩增反应，该制剂必须在热和机械上均稳定。在这里，我们对适用于我们以前开发的基于微毛细管阵列（MiCA）的离心乳液生成方法的稳定单分散w / o乳液的油和表面活性剂成分进行了系统的调查。我们的发现表明，由棕榈酸异丙酯和有机硅共聚物组成的二元配方具有最佳性能，
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