Surfactant and oil formulations for monodisperse droplet emulsion PCR

Fangli Zhang; Peiyu Liao; Yue Sun; Zitian Chen; Yuhong Pang; Yanyi Huang

doi:10.1039/D0LC00052C

Surfactant and oil formulations for monodisperse droplet emulsion PCR†

Fangli Zhang,^ab Peiyu Liao,^ab Yue Sun,^bc Zitian Chen,^ab Yuhong Pang^bd and Yanyi Huang

*^abcefg

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* Corresponding authors

^a Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China
E-mail: yanyi@pku.edu.cn

^b Biomedical Pioneering Innovation Center (BIOPIC), Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing 100871, China

^c Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China

^d School of Life Sciences, Peking University, Beijing 100871, China

^e College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China

^f Institute for Cell Analysis, Shenzhen Bay Laboratory, Guangdong 518132, China

^g Chinese Institute for Brain Research, Beijing 102206, China

Abstract

Emulsion PCR has become a popular and widely applied method in biological research and clinical diagnostics to provide evenly amplified products and perform highly quantitative counting of target sequences. However, there is still a lack of information to support further development of appropriate water-in-oil emulsion formulations, which need to be both thermally and mechanically stable for digital amplification reactions. Here, we present a systematic survey of the oil and surfactant components of stable monodisperse w/o emulsions suitable for use with our previously developed micro-capillary array (MiCA)-based centrifugal emulsion generation method. Our findings show that a binary formula consisting of isopropyl palmitate and a silicone copolymer demonstrated the best performance, and provided a general guideline for the development of emulsion systems for digital PCR and emulsion amplification applications.

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DOI: https://doi.org/10.1039/D0LC00052C
Article type: Paper
Submitted: 16 Jan 2020
Accepted: 15 May 2020
First published: 15 May 2020

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Lab Chip, 2020,20, 2328-2333

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Surfactant and oil formulations for monodisperse droplet emulsion PCR

F. Zhang, P. Liao, Y. Sun, Z. Chen, Y. Pang and Y. Huang, Lab Chip, 2020, 20, 2328 DOI: 10.1039/D0LC00052C

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Surfactant and oil formulations for monodisperse droplet emulsion PCR†

Abstract

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Surfactant and oil formulations for monodisperse droplet emulsion PCR

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