Abstract

Effects of presowing seed treatment of Triticum aestivum L. and Tr. timopheevii Zhuk. wheat with bacteria Bacillussubtilis and B. thuringiensis were studied. Seedling growth, their generation of hydrogen peroxide, and the activities of pathogenesis-related (PR) proteins oxalate oxidase (OxO), peroxidase (PO), proteinase inhibitor (PI), and chitinase upon infection with the causal fungus of bunt Tilletia caries (DC.) Tul. were analyzed. It was found that Bacillus spp. bacteria attenuated the growth suppression of seedlings caused by the bunt infection and alleviated their affection by the pathogen. The found increase in bunt resistance was associated with the boost in the H₂O₂ level, OxO and PO activities, and transcriptional activities of the chitinase TaChiI gene initiated by Bacillus spp. bacteria. The differences in activation of transcriptional activity of the proteinase inhibitor TaPI gene in soft wheat seedlings under the influence of B. subtilis and B. thuringiensis strains and their mixture were revealed. The results suppose that the wheat resistance to Til. caries may be formed through different paths, which may be related to the nature of the producer organism.

中文翻译：

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芽孢杆菌细菌对病原菌诱导的蛋白质活性和小麦抗线虫病（DC。）的抗邦特病的影响

摘要

播种处理小麦和黑麦的效果。提莫非维·朱克（timopheevii Zhuk）。用枯草芽孢杆菌和苏云金芽孢杆菌对小麦进行了研究。感染丁香龋病的病原真菌后，幼苗的生长，过氧化氢的产生以及与病程相关的（PR）蛋白草酸氧化酶（OxO），过氧化物酶（PO），蛋白酶抑制剂（PI）和几丁质酶的活性（ DC。）被分析。发现芽孢杆菌spp。细菌减弱了由bun虫感染引起的幼苗生长抑制，并减轻了病原体对它们的影响。发现的抗短打性的增强与芽孢杆菌启动的几丁质酶TaChiI基因的H ₂ O ₂水平，OxO和PO活性增强以及转录活性有关。菌。揭示了在枯草芽孢杆菌和苏云金芽孢杆菌菌株及其混合物的影响下，软小麦幼苗中蛋白酶抑制剂TaPI基因转录活性的激活差异。结果假定小麦对Til具有抗性。龋齿 可能通过不同的途径形成，这可能与生产者生物的性质有关。