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Effects of Bacillus Bacteria on Activity of Pathogen-Induced Proteins and Wheat Resistance to Bunt Caused by Tilletia caries (DC.) Tul.

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Abstract

Effects of presowing seed treatment of Triticum aestivum L. and Tr. timopheevii Zhuk. wheat with bacteria Bacillussubtilis and B. thuringiensis were studied. Seedling growth, their generation of hydrogen peroxide, and the activities of pathogenesis-related (PR) proteins oxalate oxidase (OxO), peroxidase (PO), proteinase inhibitor (PI), and chitinase upon infection with the causal fungus of bunt Tilletia caries (DC.) Tul. were analyzed. It was found that Bacillus spp. bacteria attenuated the growth suppression of seedlings caused by the bunt infection and alleviated their affection by the pathogen. The found increase in bunt resistance was associated with the boost in the H2O2 level, OxO and PO activities, and transcriptional activities of the chitinase TaChiI gene initiated by Bacillus spp. bacteria. The differences in activation of transcriptional activity of the proteinase inhibitor TaPI gene in soft wheat seedlings under the influence of B. subtilis and B. thuringiensis strains and their mixture were revealed. The results suppose that the wheat resistance to Til. caries may be formed through different paths, which may be related to the nature of the producer organism.

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ACKNOWLEDGMENTS

The equipment of Biomika Center of Collective Use (Department of Biochemical Research and Nanobiotechnology of the Agidel Regional Center of Collective Use) and that of the CODINK Unique Scientific Installation (no. USU_73598) was used.

Funding

The work was carried out through State Task no. AAA-A16-116020350027-7.

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Authors and Affiliations

  1. Ufa Institute of Biochemistry and Genetics, Ufa Federal Research Center, Russian Academy of Sciences, 450054, Ufa, Bashkortostan, Russia

    L. G. Yarullina & G. F. Burkhanova

  2. Bashkir State University, 450076, Ufa, Bashkortostan, Russia

    L. G. Yarullina & V. O. Tsvetkov

Authors
  1. L. G. Yarullina
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  2. G. F. Burkhanova
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  3. V. O. Tsvetkov
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Correspondence to L. G. Yarullina.

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This article does not contain any studies involving animals or human participants performed by any of the authors.

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The authors declare that they have no conflict of interests.

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Translated by A. Aver’yanov

Abbreviations: ISR—induced systemic resistance; JA—jasmonic acid; OxO—oxalate oxidase; PI—proteinase inhibitors; PGPB—plant growth-promoting bacteria; PO—peroxidase; PR proteins—pathogenesis-related proteins; SA—salicylic acid; SB—succinate buffer.

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Yarullina, L.G., Burkhanova, G.F. & Tsvetkov, V.O. Effects of Bacillus Bacteria on Activity of Pathogen-Induced Proteins and Wheat Resistance to Bunt Caused by Tilletia caries (DC.) Tul.. Russ J Plant Physiol 67, 564–571 (2020). https://doi.org/10.1134/S1021443720030218

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  • DOI: https://doi.org/10.1134/S1021443720030218

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