The tissue distribution pattern of lipid is highly diverse among different fish species. Tiger puffer has a special lipid storage pattern, storing lipid predominantly in liver. In order to better understand the lipid physiology in fish storing lipid in liver, the present study preliminarily investigated the tissue distribution of transcription for 29 lipid metabolism-related genes in tiger puffer, which are involved in lipogenesis, fatty acid oxidation, biosynthesis and hydrolysis of glycerides, lipid transport, and relevant transcription regulation. Samples of eight tissues, brain, eye, heart, spleen, liver, intestine, skin, and muscle, from fifteen juvenile tiger puffer were used in the qRT-PCR analysis. The intestine and brain had high transcription of lipogenic genes, whereas the liver and muscle had low expression levels. The intestine also had the highest transcription level of most apolipoproteins and lipid metabolism-related transcription factors. The transcription of fatty acid β-oxidation-related genes was low in the muscle. The peroxisomal fatty acid oxidation may dominate over mitochondrial β-oxidation in the liver and intestine of tiger puffer, and the MAG pathway probably predominates over the G3P pathway in re-acylation of absorbed lipids in the intestine. The intracellular glyceridases were highly transcribed in the brain, eye, and heart. In conclusion, in tiger puffer, the intestine could be a center of lipid metabolism whereas the liver is more likely a pure storage organ for lipid. The lipid metabolism in the muscle could also be inactive, possibly due to the very low level of intramuscular lipid.

中文翻译：

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Takifugu rubripes（一种主要在肝脏中存储脂质的海洋硬骨鱼）中29种脂质代谢相关基因的转录组织分布。

在不同鱼类中，脂质的组织分布模式高度不同。老虎河豚有特殊的脂质存储模式，主要在肝脏中存储脂质。为了更好地了解鱼类肝脏中储存脂质的脂质生理，本研究初步研究了老虎河豚体内29个脂质代谢相关基因的转录组织分布，这些基因与脂肪的产生，脂肪酸的氧化，生物合成和水解有关。甘油酯，脂质转运和相关的转录调控。qRT-PCR分析使用了十五只老虎幼崽的八种组织（脑，眼，心脏，脾，肝，肠，皮肤和肌肉）的样本。肠道和大脑的脂肪生成基因转录较高，而肝脏和肌肉的表达水平较低。在大多数载脂蛋白和脂质代谢相关的转录因子中，肠的转录水平也最高。肌肉中脂肪酸β-氧化相关基因的转录较低。过氧化物酶体脂肪酸的氧化作用可能超过老虎和河豚肝脏和肠道中的线粒体β-氧化作用，并且在肠道内吸收脂质的再酰化过程中，MAG途径可能比G3P途径占优势。细胞内甘油酸酶在脑，眼和心脏中高度转录。总之，在河豚中，肠道可能是脂质代谢的中心，而肝脏更可能是脂质的纯储存器官。肌肉中的脂质代谢也可能不活跃，可能是由于肌内脂质的水平非常低。