Abstract
The tissue distribution pattern of lipid is highly diverse among different fish species. Tiger puffer has a special lipid storage pattern, storing lipid predominantly in liver. In order to better understand the lipid physiology in fish storing lipid in liver, the present study preliminarily investigated the tissue distribution of transcription for 29 lipid metabolism-related genes in tiger puffer, which are involved in lipogenesis, fatty acid oxidation, biosynthesis and hydrolysis of glycerides, lipid transport, and relevant transcription regulation. Samples of eight tissues, brain, eye, heart, spleen, liver, intestine, skin, and muscle, from fifteen juvenile tiger puffer were used in the qRT-PCR analysis. The intestine and brain had high transcription of lipogenic genes, whereas the liver and muscle had low expression levels. The intestine also had the highest transcription level of most apolipoproteins and lipid metabolism-related transcription factors. The transcription of fatty acid β-oxidation-related genes was low in the muscle. The peroxisomal fatty acid oxidation may dominate over mitochondrial β-oxidation in the liver and intestine of tiger puffer, and the MAG pathway probably predominates over the G3P pathway in re-acylation of absorbed lipids in the intestine. The intracellular glyceridases were highly transcribed in the brain, eye, and heart. In conclusion, in tiger puffer, the intestine could be a center of lipid metabolism whereas the liver is more likely a pure storage organ for lipid. The lipid metabolism in the muscle could also be inactive, possibly due to the very low level of intramuscular lipid.
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Abbreviations
- acacβ :
-
Acetyl-CoA carboxylase beta
- fas :
-
Fatty acid synthase
- scd1 :
-
Delta-9-desaturase 1
- acox1 :
-
Acyl-CoA oxidase 1, palmitoyl
- acox3 :
-
Acyl-CoA oxidase 3, pristanoyl
- cpt-1 :
-
Carnitine O-palmitoyltransferase-1
- gpat :
-
Glycerol-3-phosphate acyltransferase
- dgat1:
-
Diacylglycerol O-acyltransferase 1
- bsal :
-
Bile acid-activated lipase
- hsl :
-
Hormone-sensitive lipase
- daglα :
-
Diacylglycerol lipase, alpha
- mgll :
-
Monoglyceride lipase
- lpl :
-
Lipoprotein lipase
- lipc :
-
Lipase, hepatic
- apo :
-
Apolipoprotein
- mttp :
-
Microsomal triglyceride transfer protein
- srebf1 :
-
Sterol regulatory element binding transcription factor 1
- ppar :
-
Peroxisome proliferators-activated receptor
- fxr :
-
Farnesoid X receptor (nuclear receptor subfamily 1, group H, member 4, NR1H4)
- lxrα:
-
Liver X receptor alpha (nuclear receptor subfamily 1, group H, member 3, NR1H3)
- hnf4α :
-
Hepatocyte nuclear factor 4, alpha
- lrh-1 :
-
Liver receptor homolog-1 (nuclear receptor subfamily 5, group A, member 2, NR5A2)
- gys2 :
-
Glycogen synthase 2
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Funding
This work was supported by the Central Public-interest Scientific Institution Basal Research Fund, CAFS (2018HY-ZD0505, 2020TD48), the National Key R&D Program of China (2018YFD0900400), the National Natural Science Foundation of China (31972803), and China Agriculture Research System (CARS-47-G15).
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Xu, H., Meng, X., Jia, L. et al. Tissue distribution of transcription for 29 lipid metabolism-related genes in Takifugu rubripes, a marine teleost storing lipid predominantly in liver. Fish Physiol Biochem 46, 1603–1619 (2020). https://doi.org/10.1007/s10695-020-00815-7
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DOI: https://doi.org/10.1007/s10695-020-00815-7