The quest to replace animal models used in drug testing owing to their lack of accuracy in reflecting human physiology, and the higher comparative cost and time involved in testing with such animal models has given rise to the organ-on-a-chip technology. Organ-on-a-chip-based microphysiological systems are flexible and can be engineered to specifically mimic desired organs and tissue types for the drug discovery and development process. Kidney-specific and non-specific drugs either directly or indirectly affect the kidneys’ function by inducing kidney injury. It is quite challenging to integrate electrochemical sensors in the microphysiological systems for continuous monitoring of micro-environment metabolism. We present a theranostic proximal tubule-on-a-chip model for live monitoring of cellular growth pattern. The sensors monitored real-time changes under disease condition and drug treatment based upon cell adhesion and culture medium pH. A glass-based microfluidic chip was designed with integrated transparent electrodes for transepithelial electrical resistance (TEER) monitoring. Additionally, an optical pH sensor and a microscope have been added in the platform for the real-time monitoring of the tissue. This model has the potential to study the absorption and metabolism of the drug along with the capacity to complete and optimize its toxicity assessment.

由于缺乏反映人类生理的准确性，以及替代药物测试所用的动物模型，以及使用这种动物模型进行测试所涉及的较高的比较成本和时间，寻求替代动物芯片技术已经引起了人们的关注。基于芯片上器官的微生理系统非常灵活，可以进行工程设计以专门模拟所需的器官和组织类型，以进行药物发现和开发过程。肾脏特异性和非特异性药物通过诱导肾脏损伤直接或间接影响肾脏功能。将电化学传感器集成到微生理系统中以连续监测微环境代谢是非常具有挑战性的。我们提出了一个用于治疗细胞生长模式的实时诊断的近端肾小管上片模型。传感器根据细胞粘附和培养基pH值，监测疾病状况和药物治疗下的实时变化。设计了带有集成透明电极的玻璃基微流控芯片，用于跨上皮电阻（TEER）监测。此外，平台中还增加了光学pH传感器和显微镜，用于组织的实时监控。该模型具有研究药物的吸收和代谢以及完成和优化其毒性评估的能力。平台中增加了光学pH传感器和显微镜，用于组织的实时监控。该模型具有研究药物的吸收和代谢以及完成和优化其毒性评估的能力。平台中增加了光学pH传感器和显微镜，用于组织的实时监控。该模型具有研究药物的吸收和代谢以及完成和优化其毒性评估的能力。