Stoichiometric control of input substrate (glucose) and native soil organic C (SOC) mineralization was assessed by performing a manipulation experiment based on N or P fertilization in paddy soil. Glucose mineralization increased with nutrient addition up to 11.6% with combined N and P application compared with that without nutrient addition. During 100 days of incubation, approximately 4.5% of SOC was mineralized and was stimulated by glucose addition. Glucose and SOC mineralization increased exponentially with dissolved organic C (DOC):NH4+-N, DOC:Olsen P, and microbial biomass (MB)C:MBN ratios. The relative abundances of Clostridia and β-Proteobacteria (r-strategists) were increased with combined C and NP application at the beginning of the experiment, while the relative abundances of Acidobacteria (K-strategists) were enhanced with the exhaustion of available resource at the end of incubation. The bacteria abundance and diversity were negatively related to the DOC:NH4+-N and DOC:Olsen P, which had direct positive effects (+ 0.63) on SOC mineralization. Combined glucose and NP application decreased the network density of the bacterial community. Moreover, P addition significantly decreased the negative associations among bacterial taxa, which suggested that microbial competition for nutrients was alleviated. The relative abundances of keystone species showed significant positive correlations with SOC mineralization in the soils without P application, revealing that microbes increased their activity for mining of limited nutrients from soil organic matter. Hence, bacteria shifted their community composition and their interactions to acquire necessary elements by increasing SOC mineralization to maintain the microbial biomass C:N:P stoichiometric balance in response to changes in resource stoichiometry.

中文翻译：

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C:N:P 化学计量调节土壤有机碳矿化和水稻土壤微生物群落组成的伴随变化

输入底物（葡萄糖）和原生土壤有机碳 (SOC) 矿化的化学计量控制通过基于水稻土壤中的 N 或 P 施肥进行操作实验来评估。与不添加养分相比，氮磷联合施用使葡萄糖矿化增加了11.6%。在 100 天的孵化过程中，大约 4.5% 的 SOC 被矿化并受到添加葡萄糖的刺激。葡萄糖和 SOC 矿化随溶解有机碳 (DOC):NH4+-N、DOC:Olsen P 和微生物生物量 (MB)C:MBN 比率呈指数增长。梭菌和 β-变形菌（r-策略者）的相对丰度随着实验开始时 C 和 NP 的组合应用而增加，而酸杆菌（K-策略）的相对丰度随着孵化结束时可用资源的枯竭而增加。细菌丰度和多样性与 DOC:NH4+-N 和 DOC:Olsen P 呈负相关，对 SOC 矿化有直接的积极影响（+ 0.63）。葡萄糖和 NP 的组合应用降低了细菌群落的网络密度。此外，磷的添加显着降低了细菌分类群之间的负相关，这表明微生物对营养物质的竞争得到了缓解。在未施磷的土壤中，关键物种的相对丰度与 SOC 矿化呈显着正相关，表明微生物增加了从土壤有机质中挖掘有限养分的活性。因此，