Double-stranded RNA and total RNA purified from sour cherry leaves (Prunus cerasus, cv. Amarelka Chvalkovicka) was analyzed by high-throughput sequencing. BLAST annotation identified contigs with homology to several already known cherry-infecting viruses (prune dwarf virus, prunus necrotic ringspot virus, prunus virus F, little cherry virus 1) as well as contigs with sequences more distantly related to those of members of the family Betaflexiviridae and in particular to prunus virus T of the genus Tepovirus. The full genome sequence of a putative virus (6,847 nucleotides [nt]; GenBank no. MT090966) was assembled and completed at the genome ends. The genome has a typical tepovirus organization, containing three overlapping open reading frames (ORFs), encoding a replication-associated protein, a movement protein and a capsid protein, respectively. Both its genome organization and its phylogenetic relationships show that the virus belongs to the genus Tepovirus, but considering the species demarcation criteria for the family Betaflexiviridae, it appears to represent a novel virus species, and we propose the name "cherry virus T" (ChVT) for this virus.

中文翻译：

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樱桃病毒 T 的完整基因组序列，一种新型感染樱桃的丁病毒。

通过高通量测序分析从酸樱桃叶（Prunus cerasus，cv. Amarelka Chvalkovicka）中纯化的双链 RNA 和总 RNA。BLAST 注释鉴定出与几种已知的樱桃感染病毒（李子矮病毒、李子坏死环斑病毒、李子病毒 F、小樱桃病毒 1）具有同源性的重叠群，以及与 Betaflexiviridae 家族成员的序列关系更远的重叠群特别是Tepovirus属的李属病毒T。推定病毒的完整基因组序列（6,847 个核苷酸 [nt]；GenBank 编号 MT090966）在基因组末端组装并完成。基因组具有典型的替波病毒组织，包含三个重叠的开放阅读框（ORF），分别编码复制相关蛋白、运动蛋白和衣壳蛋白。其基因组结构和系统发育关系均表明该病毒属于Tepovirus属，但考虑到Betaflexiviridae科的物种划分标准，它似乎代表了一种新的病毒物种，我们建议将其命名为“樱桃病毒T”（ChVT） ）对于这种病毒。