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A new begomovirus isolated from a potyvirus-infected bean plant causes asymptomatic infections in bean and N. benthamiana.
Archives of Virology ( IF 2.5 ) Pub Date : 2020-05-13 , DOI: 10.1007/s00705-020-04646-y
Nadia Martínez-Marrero 1 , Jesús Aarón Avalos-Calleros 1 , Elizabeth Chiquito-Almanza 2 , Jorge Alberto Acosta-Gallegos 2 , Salvador Ambriz-Granados 1 , José Luis Anaya-López 2 , Gerardo Rafael Argüello-Astorga 1
Affiliation  

In this work, a begomovirus isolated from a bean plant coinfected with the potyviruses bean common mosaic virus and bean common mosaic necrosis virus was characterized. The three viruses were detected by high-throughput sequencing and assembly of total small RNAs, but the begomovirus-related contigs did not allow precise identification. Molecular analysis based on standard DNA amplification techniques revealed the presence of a single bipartite virus, which is a novel begomovirus according to the current taxonomic criteria. Infectious clones were generated and agroinoculated into Phaseolus vulgaris and Nicotiana benthamiana plants. In all cases, viral DNA-A and DNA-B were detected in new growths, but no symptoms were observed, thus indicating that this virus produces asymptomatic infections in both host species.

中文翻译:


从感染马铃薯Y病毒的豆类植物中分离出一种新的贝戈莫病毒,可引起豆类和本塞姆氏烟草的无症状感染。



在这项工作中,对从与马铃薯病毒属豆常见花叶病毒和豆常见花叶坏死病毒共感染的豆类植物中分离出的begomovirus进行了表征。通过高通量测序和总小RNA组装检测到这三种病毒,但与begomovirus相关的重叠群无法进行精确识别。基于标准DNA扩增技术的分子分析揭示了单一二分病毒的存在,根据当前的分类标准,这是一种新型的贝戈莫病毒。产生感染性克隆并将其农杆菌接种到菜豆和本塞姆氏烟草植物中。在所有病例中,在新生长物中均检测到病毒 DNA-A 和 DNA-B,但没有观察到任何症状,因此表明该病毒在两种宿主物种中产生无症状感染。
更新日期:2020-05-13
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