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A new begomovirus isolated from a potyvirus-infected bean plant causes asymptomatic infections in bean and N. benthamiana

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Abstract

In this work, a begomovirus isolated from a bean plant coinfected with the potyviruses bean common mosaic virus and bean common mosaic necrosis virus was characterized. The three viruses were detected by high-throughput sequencing and assembly of total small RNAs, but the begomovirus-related contigs did not allow precise identification. Molecular analysis based on standard DNA amplification techniques revealed the presence of a single bipartite virus, which is a novel begomovirus according to the current taxonomic criteria. Infectious clones were generated and agroinoculated into Phaseolus vulgaris and Nicotiana benthamiana plants. In all cases, viral DNA-A and DNA-B were detected in new growths, but no symptoms were observed, thus indicating that this virus produces asymptomatic infections in both host species.

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Acknowledgments

We would like to thank the National Laboratory LANBAMA (IPICYT) for the technical assistance provided. NMM, JAAC, and ECA were supported by a fellowship from the Consejo Nacional de Ciencia y Tecnologıa (CONACYT, México).

Funding

This study was funded by Consejo Nacional de Ciencia y Tecnología (CONACYT, Mexico), Grants: PDCPN 2013-1 No. 214641 and CB 2015-257136-B; and INIFAP, México. Project No. 1551434856.

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Authors and Affiliations

  1. División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, C.P. 78216, San Luis Potosí, San Luis Potosí, México

    Nadia Martínez-Marrero, Jesús Aarón Avalos-Calleros, Salvador Ambriz-Granados & Gerardo Rafael Argüello-Astorga

  2. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Camino a la Presa San Jose 2055, C.P. 38110, San Luis Potosi, San Luis Potosi, México

    Elizabeth Chiquito-Almanza, Jorge Alberto Acosta-Gallegos & José Luis Anaya-López

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  1. Nadia Martínez-Marrero
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  2. Jesús Aarón Avalos-Calleros
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  3. Elizabeth Chiquito-Almanza
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Correspondence to Gerardo Rafael Argüello-Astorga.

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Martínez-Marrero, N., Avalos-Calleros, J.A., Chiquito-Almanza, E. et al. A new begomovirus isolated from a potyvirus-infected bean plant causes asymptomatic infections in bean and N. benthamiana. Arch Virol 165, 1659–1665 (2020). https://doi.org/10.1007/s00705-020-04646-y

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  • DOI: https://doi.org/10.1007/s00705-020-04646-y

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