In vitro bioactivity, biodegradation, and biocompatibility behavior of a new bioactive glass systems were investigated with the incorporation of selenium oxide, for the replacement of sodium oxide in the traditional 45S5 bioglass composition. The apatite-forming ability of melt-derived bioactive glasses was evaluated by immersion studies in simulated body fluid while monitoring the concentration of silicon, calcium, phosphorus, sodium, and selenium in the medium. The weight loss of bioactive glasses and pH change in the tris-(hydroxymethyl)-amino methane buffer solution was determined to observe the biodegradation behavior of glass samples. The glasses were characterized by a Fourier transform infrared spectroscopy, scanning electron microscopy, inductively coupled plasma, and Vickers hardness measurements. The biocompatibility evaluation of the glasses was determined through in vitro osteogenesis assays by cell viability, alkaline phosphatase activity, and mineralized matrix formation. The incorporation of selenium enhanced the hydroxyapatite formation on the bioactive glass surface and microhardness of glasses. The hardness of glasses was found to decrease with immersion duration. The results indicate that selenium incorporated bioactive glasses can be used as bioactive material in bone tissue engineering applications.

中文翻译：

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硒掺入对45S5生物玻璃结构和体外生物活性的影响

研究了新型生物活性玻璃系统的体外生物活性，生物降解性和生物相容性行为，并结合了氧化硒，以代替传统45S5生物玻璃组合物中的氧化钠。通过在模拟体液中浸泡研究，同时监测培养基中硅，钙，磷，钠和硒的浓度，评估了熔融衍生生物活性玻璃的磷灰石形成能力。测定生物活性玻璃的重量损失和三-（羟甲基）-氨基甲烷缓冲溶液中的pH变化，以观察玻璃样品的生物降解行为。通过傅里叶变换红外光谱，扫描电子显微镜，电感耦合等离子体和维氏硬度测量来表征玻璃。通过细胞成活，碱性磷酸酶活性和矿化基质的形成，通过体外成骨试验确定了玻璃的生物相容性。硒的掺入增强了生物活性玻璃表面上羟基磷灰石的形成和玻璃的显微硬度。发现玻璃的硬度随着浸泡时间的延长而降低。结果表明，掺硒的生物活性玻璃可用作骨组织工程应用中的生物活性材料。发现玻璃的硬度随着浸泡时间的延长而降低。结果表明，掺硒的生物活性玻璃可用作骨组织工程应用中的生物活性材料。发现玻璃的硬度随着浸泡时间的延长而降低。结果表明，掺硒的生物活性玻璃可用作骨组织工程应用中的生物活性材料。