This paper reports a study of biofilm formation by the Mycoplasma pneumoniae (Mp) strain Fh on an abiotic surface using light and electron microscopy. Quantitative assessment of the effectiveness of biofilm formation was carried out in polystyrene plates with crystal-Violet cell staining. To study the ultrastructural organization of the Mp cells, scanning electron microscopy was used, which allowed a well-formed multicellular clearly defined structure of considerable size to be revealed by the third day of culturing in which Mp cells were submerged into the extracellular matrix with a clearly detectable system of pores. The morphology of the detected aggregations was absolutely identical to that of the biofilms described for other microorganisms, which confirmed that Mp had the ability to to form biofilms. The described phenomenon of the ability of Mp to grow in the form of the biofilm is of great importance both for understanding the causes of the long-term persistence of Mp in the human organism and for the development of new approaches for the treatment of protracted and chronic forms of infectious diseases caused by Mp.

中文翻译：

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呼吸道支原体病病原体肺炎支原体的体外生物膜形成

本文报告了使用光学和电子显微镜对非生物表面上的肺炎支原体 (Mp) 菌株 Fh 生物膜形成的研究。在具有结晶紫细胞染色的聚苯乙烯板中进行生物膜形成有效性的定量评估。为了研究 Mp 细胞的超微结构组织，使用了扫描电子显微镜，在培养的第三天，Mp 细胞被用清晰可检测的孔隙系统。检测到的聚集体的形态与其他微生物所描述的生物膜的形态完全相同，这证实了 Mp 具有形成生物膜的能力。