Abstract

The objective of the investigation was to study variation of storage proteins in two Crimean populations of Dasypyrum villosum from Beregove of the Bakhchisarai region and from the Tauric Chersonese National Reserve (Sevastopol). Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis of seed storage proteins was performed to analyze diversity of high-molecular-weight glutenin subunits encoded by the Glu-V1 locus, as well as ω-gliadin variants on SDS-electrophoregrams encoded by Gli-V1. In the two Crimean populations of D. villosum, eight alleles at the Glu-V1 locus and four Gli-V1 alleles encoding ω-gliadin were identified. The Crimean populations of D. villosum differed significantly in the frequencies of the alleles a, b, and c at the Glu-V1 locus and both had a high frequency of the null allele (k). The populations showed significant differences in frequencies of ω-gliadin variants on SDS-electrophoregrams encoded by Gli-V1, and gene diversity with respect to this marker was higher in the population of Beregove than that in the Chersonese population. ω-Gliadins on SDS-electrophoregrams are a convenient marker system for analysis of D. villosum populations, which can be employed simultaneously with analysis of high-molecular-weight glutenin subunits.

中文翻译：

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稻瘟病菌克里米亚种群中贮藏蛋白的变化

摘要

该研究的目的是研究来自Bakhchisarai地区的Beregove和Tauric Chersonese国家自然保护区（塞瓦斯托波尔）的两个克里希亚绒毛菊花种群的贮藏蛋白变异。进行种子存储蛋白的十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶电泳以分析Glu-V1基因座编码的高分子量谷蛋白亚基的多样性以及Gli-V1编码的SDS电泳图上的ω-麦醇溶蛋白变体。在两个D. villosum克里米亚人口中，确定了在Glu-V1位点的八个等位基因和四个编码ω-麦醇溶蛋白的Gli-V1等位基因。的克里米亚人口D. villosum在Glu-V1位点的等位基因a，b和c的频率有显着差异，并且都具有较高的无效等位基因（k）的频率。在Gli-V1编码的SDS电泳图上，这些种群的ω-麦醇溶蛋白变体的频率显示出显着差异，在Beregove种群中，与该标记有关的基因多样性高于在Chersonese种群中。ω - SDS电泳图上的麦醇溶蛋白是一种方便的标记系统，用于分析绒毛木犀人群，可以与高分子量谷蛋白亚基的分析同时使用。