Dexmedetomidine has been confirmed to have anti-inflammatory effect in numerous diseases. However, the detailed mechanism is still unknown. We explore the hypothesis that CD200R is related to the anti-inflammatory effect of dexmedetomidine. Primary microglia were treated with different concentrations of dexmedetomidine followed with lipopolysaccharide (LPS) for 24 h. Interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10) were measured by enzyme-linked immunosorbent assay. Arginase 1 (Arg1), IL-6, and TNF-α were measured by TR-PCR. CD200R was observed by Western blot analysis. We found dexmedetomidine reduced LPS-induced IL-6 and TNF-α production and increase Arg1 in primary microglia. CD200R expression was downregulated by LPS and dexmedetomidine can attenuate the effect of LPS. Our results showed that CD200R may be related to the anti-inflammatory effect of dexmedetomidine.

中文翻译：

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CD200R参与右旋美托咪定在脂多糖刺激的小胶质细胞中的抗炎作用。

右美托咪定已被证实在多种疾病中具有抗炎作用。但是，详细的机制仍然未知。我们探索CD200R与右美托咪定的抗炎作用有关的假说。原发性小胶质细胞先用不同浓度的右美托咪定处理，再用脂多糖（LPS）处理24小时。用酶联免疫吸附法测定白介素-6（IL-6），肿瘤坏死因子-α（TNF-α）和白介素-10（IL-10）。通过TR-PCR测量精氨酸酶1（Arg1），IL-6和TNF-α。通过蛋白质印迹分析观察到CD200R。我们发现右美托咪定减少原发性小胶质细胞中LPS诱导的IL-6和TNF-α的产生并增加Arg1。LPS下调CD200R的表达，右美托咪定可以减弱LPS的作用。