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CD200R Is Involved in the Anti-inflammatory Effect of Dexmedetomidine in Lipopolysaccharide-Stimulated Microglia

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Abstract

Dexmedetomidine has been confirmed to have anti-inflammatory effect in numerous diseases. However, the detailed mechanism is still unknown. We explore the hypothesis that CD200R is related to the anti-inflammatory effect of dexmedetomidine. Primary microglia were treated with different concentrations of dexmedetomidine followed with lipopolysaccharide (LPS) for 24 h. Interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10) were measured by enzyme-linked immunosorbent assay. Arginase 1 (Arg1), IL-6, and TNF-α were measured by TR-PCR. CD200R was observed by Western blot analysis. We found dexmedetomidine reduced LPS-induced IL-6 and TNF-α production and increase Arg1 in primary microglia. CD200R expression was downregulated by LPS and dexmedetomidine can attenuate the effect of LPS. Our results showed that CD200R may be related to the anti-inflammatory effect of dexmedetomidine.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (81701050), Science and Technology Development Fund of Nanjing Medical University (NMUB2018259), Jiangsu Planned Projects for Postdoctoral Research Funds (2018K075B), Six talent peaks project in Jiangsu Province (WSW-329, YY124), Six one project in Jiangsu Province (LGY2018049, LYG2019061), and Open Project Program of Jiangsu Key Laboratory for Anesthesiology (201922).

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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Xiaobao Zhang, Jizheng Cui, Haitao Qian, Bing Wang, Zhibin Zhao, and Fang Yan. The first draft of the manuscript was written by Xiaobao Zhang and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Fang Yan or Zhibin Zhao.

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Zhang, X., Cui, J., Qian, H. et al. CD200R Is Involved in the Anti-inflammatory Effect of Dexmedetomidine in Lipopolysaccharide-Stimulated Microglia. Inflammation 43, 1707–1715 (2020). https://doi.org/10.1007/s10753-020-01244-7

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