Ocimum basilicum L. var purpurascens (purple basil) contains medicinally valuable metabolites. Light greatly influences the physiological processes, including biomass accumulation and secondary metabolites production in medicinal plants. Herein, we investigated the influence of different spectral lights on the biosynthesis of phenylpropanoid metabolites in purple basil callus cultures. Growth kinetics was østudied for a total of 49 days, with 7 days of sampling time. Among the various treatments, blue light resulted in maximum biomass accumulation, total phenolic content (TPC), total flavonoid content (TFC) and antioxidant DPPH, FRAP and ABTS activities, as compared to controls. Moreover, blue light also encouraged higher superoxide dismutase activity while the red light was found effective for enhanced peroxidase activity. HPLC analysis revealed enhanced rosmarinic acid (87.62 mg/g DW) and anthocyanins (cyanidin: 0.15 mg/g DW and peonidin: 0.13 mg/g DW) contents under dark grown callus cultures which were almost 1.55, 1.25 and 1.18–fold greater than controls, respectively. Conversely, red light caused maximum production of cichoric acid (14.65 mg/g DW). Moreover, a positive correlation occurred among the accumulation of phenolic and flavonoids and antioxidant activities. These results suggest that light quality strongly influences medicinally valuable phenylpropanoid metabolites biosynthesis along with antioxidant potential in in vitro cultures of purple basil.

罗勒叶紫罗兰（紫色罗勒）含有可药用的代谢物。光极大地影响了生理过程，包括药用植物中生物量的积累和次生代谢产物的产生。在这里，我们研究了不同的光谱光对紫色罗勒愈伤组织培养物中苯丙烷代谢产物生物合成的影响。总共研究了49天的生长动力学，其中采样时间为7天。与对照相比，在各种处理中，蓝光导致最大的生物量积累，总酚含量（TPC），总黄酮含量（TFC）和抗氧化剂DPPH，FRAP和ABTS活性。此外，蓝光还鼓励更高的超氧化物歧化酶活性，而红光被发现对于增强过氧化物酶活性有效。HPLC分析显示增强了迷迭香酸（87。在黑暗生长的愈伤组织培养下，其花青素含量为62 mg / g DW）和花青素（花青素：0.15 mg / g DW和皮苷：0.13 mg / g DW），分别比对照高近1.55、1.25和1.18倍。相反，红光引起最大的胆酸产生（14.65 mg / g DW）。此外，酚类和类黄酮的积累与抗氧化活性之间存在正相关。这些结果表明，光质量强烈影响紫罗勒离体培养中具有药用价值的苯丙烷类代谢物的生物合成以及抗氧化能力。红光引起最大的胆酸产生（14.65 mg / g DW）。此外，酚类和类黄酮的积累与抗氧化活性之间存在正相关。这些结果表明，光质量强烈影响紫罗勒离体培养中具有药用价值的苯丙烷类代谢物的生物合成以及抗氧化能力。红光引起最大的胆酸产生（14.65 mg / g DW）。此外，酚类和类黄酮的积累与抗氧化活性之间存在正相关。这些结果表明，光质量强烈影响紫罗勒离体培养中具有药用价值的苯丙烷类代谢物的生物合成以及抗氧化能力。