The ADNMED (Anaerobic Digestion, Nitrification, and Mixotrophic Endogenous Denitrification) system comprises a triple chamber configuration that was shown to provide high-quality effluent regarding carbon, nitrogen, and sulfide. Hydraulic retention time (HRT) was 7 h in the anaerobic and anoxic chambers, and 5 h in the aerobic chamber (stage A). Sewage was directly added to the anoxic chamber to provide extra organic electron donors for denitrification (stage B) to improve the nitrogen removal efficiency (stage A 47 ± 19%). The addition of sewage at a flow rate equivalent to 10% of the feed flow increased nitrogen removal efficiency to 61 ± 12%. Illumina® sequencing revealed a restructuring of the microbial community in the anoxic chamber, according to the availability of the endogenous electron donors for denitrification. At stage A, denitrification was related to the decay of biomass, while the addition of sewage during stage B stimulated the establishment of fermentative bacteria.

中文翻译：

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水力停留时间和内源性电子供体可用性变化下的缺氧微生物群落健壮性。

ADNMED（厌氧消化，硝化和混合营养的内源性反硝化）系统包括三室配置，已显示可提供有关碳，氮和硫化物的高质量废水。在厌氧和缺氧室内，水力停留时间（HRT）为7小时，在有氧室内（A级），水力停留时间为5小时。将污水直接添加到缺氧室中，以提供额外的有机电子给体用于反硝化（阶段B），以提高脱氮效率（阶段A 47±19％）。以等于进料流量的10％的流量添加污水，可以将脱氮效率提高到61±12％。根据可用于反硝化的内源电子供体的可用性，Illumina®测序揭示了缺氧室内微生物群落的重组。在阶段A，