Tyrosinase (T3824, Sigma) enzymes were immobilized onto SnO2: Sb thin films (ATO-TF) which were synthesized in laboratory conditions by spray pyrolysis technique. Immobilization of tyrosinase onto SnO2: Sb thin film (ATO-TF) was confirmed by scanning electron microscopy (SEM) and Fourier transformed infrared spectroscopy (FTIR). The optimum pH of the immobilized tyrosinase (tyrosinase-ATO-TF) was 6.0, and the optimum temperature was found to be 30 °C. In the presence of catechol substrate of immobilized enzymes, Km and Vmax values were determined as 0.34 mM and 312.5 U/cm2 min, respectively. The thermal stabilities of the immobilized tyrosinase at 4 °C and 30 °C were investigated for 20, 40, and 60 min. At these temperatures and time intervals, the immobilized tyrosinase was found to be highly stable. The pH stability of the immobilized tyrosinase enzymes was incubated for 24, 48, 72, and 96 h at 4 °C temperature. The enzyme activity was determined under optimum conditions, and the activity of the immobilized tyrosinase enzymes exhibited various values for the range of pH 3.0-8.0. The storage stability of the immobilized tyrosinase enzymes at 4 °C was investigated, and 45.72% of the initial activity was maintained at the end of the seventh day. Furthermore, the reusability of the immobilized tyrosinase enzymes has been examined. The immobilized tyrosinase enzymes can be used 3 times and survived their 50% of initial activity. The ATO-TF can be used as alternative support materials for the immobilization of tyrosinase enzymes.

中文翻译：

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酪氨酸酶固定化的创新支持材料：掺锑氧化锡薄膜（ATO-TF）。

将酪氨酸酶（T3824，Sigma）酶固定在SnO2：Sb薄膜（ATO-TF）上，该薄膜在实验室条件下通过喷雾热解技术合成。酪氨酸酶固定在SnO2：Sb薄膜（ATO-TF）上通过扫描电子显微镜（SEM）和傅立叶变换红外光谱（FTIR）得以确认。固定化酪氨酸酶（酪氨酸酶-ATO-TF）的最适pH为6.0，最适温度为30℃。在固定化酶的邻苯二酚底物存在下，Km和Vmax值分别确定为0.34 mM和312.5 U / cm2 min。研究了固定化酪氨酸酶在4°C和30°C下20、40和60分钟的热稳定性。在这些温度和时间间隔下，发现固定化的酪氨酸酶是高度稳定的。固定化酪氨酸酶的pH稳定性在4°C下孵育24、48、72和96 h。在最佳条件下测定酶活性，并且固定的酪氨酸酶的活性在pH 3.0-8.0的范围内表现出各种值。研究了固定化酪氨酸酶在4°C下的储存稳定性，并在第7天结束时保持了45.72％的初始活性。此外，已经检查了固定化酪氨酸酶的可重复使用性。固定化的酪氨酸酶可以使用3次，并在其50％的初始活性下存活。ATO-TF可用作固定化酪氨酸酶的替代支持材料。在最佳条件下测定酶活性，并且固定的酪氨酸酶的活性在pH 3.0-8.0的范围内显示出各种值。研究了固定化酪氨酸酶在4°C下的储存稳定性，并在第7天结束时保持了45.72％的初始活性。此外，已经检查了固定化酪氨酸酶的可重复使用性。固定化的酪氨酸酶可以使用3次，并在其50％的初始活性下存活。ATO-TF可用作固定化酪氨酸酶的替代支持材料。在最佳条件下测定酶活性，并且固定的酪氨酸酶的活性在pH 3.0-8.0的范围内表现出各种值。研究了固定化酪氨酸酶在4°C下的储存稳定性，并在第7天结束时保持了45.72％的初始活性。此外，已经检查了固定化酪氨酸酶的可重复使用性。固定化的酪氨酸酶可以使用3次，并在其50％的初始活性下存活。ATO-TF可用作固定化酪氨酸酶的替代支持材料。在第七天结束时，保持了初始活动的72％。此外，已经检查了固定化酪氨酸酶的可重复使用性。固定化的酪氨酸酶可以使用3次，并在其50％的初始活性下存活。ATO-TF可用作固定化酪氨酸酶的替代支持材料。在第七天结束时，保持了初始活动的72％。此外，已经检查了固定化酪氨酸酶的可重复使用性。固定化的酪氨酸酶可以使用3次，并在其50％的初始活性下存活。ATO-TF可用作固定化酪氨酸酶的替代支持材料。