Drought stress, as a key negative factor, could result in the reduction of yield and quality in rice. Myo-inositol oxygenase (MIOX), a unique monooxygenase, exerts an essential role in plant drought resistance. To investigate the function of the OsMIOX gene in drought process, a transcriptomic profile between OsMIOX-overexpressing (OE) plants and wild-type (WT) was performed by RNA-sequencing in rice. Under drought stress, a total of 23 million high-quality reads, assembled into 28,552 unigenes, were generated and 743 genes (534 up-regulated and 209 down-regulated) were significantly differentially expressed between OE plants and WT. These detected genes were mainly associated with plant hormones transduction and sugar metabolism. Interestingly, several transcription factors (TFs) were prominently identified in OE lines. A significant positive correlation between RNA-Seq data and qRT-PCR results was identified. In all, our results could partially provide relatively valuable clues in identifying candidate genes involved in drought-stress resistance and elucidating basis theory underlying the role of OsMIOX in drought resistance.

中文翻译：

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转录组学分析表明，OsMIOX通过调节植物激素和糖相关基因的表达来提高水稻的抗旱性

干旱胁迫是主要的不利因素，可能会导致水稻产量和品质下降。肌醇加氧酶（MIOX）是一种独特的单加氧酶，在植物抗旱性中起着至关重要的作用。调查的功能OsMIOX基因在抗旱过程中，之间的转录轮廓OsMIOX在水稻中通过RNA测序进行了超表达（OE）植物和野生型（WT）植物的表达。在干旱胁迫下，共生成了2300万个高质量读段，组装成28552个单基因，并且OE植物和WT之间显着差异表达了743个基因（上调534个基因，下调209个基因）。这些检测到的基因主要与植物激素转导和糖代谢有关。有趣的是，在OE系中突出鉴定了几种转录因子（TF）。RNA-Seq数据与qRT-PCR结果之间存在显着正相关。总之，我们的结果可以部分提供相对有价值的线索，以鉴定参与抗旱性的候选基因，并阐明作为OsMIOX作用基础的基础理论 在抗旱方面。