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Transcriptomics analyses reveal that OsMIOX improves rice drought tolerance by regulating the expression of plant hormone and sugar related genes

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Abstract

Drought stress, as a key negative factor, could result in the reduction of yield and quality in rice. Myo-inositol oxygenase (MIOX), a unique monooxygenase, exerts an essential role in plant drought resistance. To investigate the function of the OsMIOX gene in drought process, a transcriptomic profile between OsMIOX-overexpressing (OE) plants and wild-type (WT) was performed by RNA-sequencing in rice. Under drought stress, a total of 23 million high-quality reads, assembled into 28,552 unigenes, were generated and 743 genes (534 up-regulated and 209 down-regulated) were significantly differentially expressed between OE plants and WT. These detected genes were mainly associated with plant hormones transduction and sugar metabolism. Interestingly, several transcription factors (TFs) were prominently identified in OE lines. A significant positive correlation between RNA-Seq data and qRT-PCR results was identified. In all, our results could partially provide relatively valuable clues in identifying candidate genes involved in drought-stress resistance and elucidating basis theory underlying the role of OsMIOX in drought resistance.

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Acknowledgements

This work was supported by the GDAS’ Project of Science and Technology Development (2019GDASYL-0103032) and the National Natural Science Foundation of China (31672097).

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Authors and Affiliations

  1. Guangdong Provincial Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute), Guangdong Engineering Research Center for Pesticide & Fertilizer, Guangzhou, 510316, China

    Fachao Shi

  2. Key Laboratory for Biological Control of the Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Yijie Dong & Dewen Qiu

  3. Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China

    Min Wang

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  1. Fachao Shi
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Correspondence to Fachao Shi.

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Supplementary material 1 (DOCX 18 kb)

11816_2020_608_MOESM2_ESM.jpg

Fig. S1. Phenotype of OsMIOX OE lines and WT plants under normal and drought conditions. (a) One month old seedlings in normal condition.(b)phenotype of plants rewatered in 3 days after 7 days drought treatment.(c) Seedlings in nutrition solution (d) the photo of seedlings without solution for three days. Supplementary material 2 (JPEG 64 kb)

11816_2020_608_MOESM3_ESM.jpg

Fig. S2.Genes expression level between WT and OsMIOX OE lines by qRT-PCR. Error bars indicate SD (n= 6). Supplementary material 3 (JPEG 110 kb)

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Shi, F., Dong, Y., Wang, M. et al. Transcriptomics analyses reveal that OsMIOX improves rice drought tolerance by regulating the expression of plant hormone and sugar related genes. Plant Biotechnol Rep 14, 339–349 (2020). https://doi.org/10.1007/s11816-020-00608-7

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